https://nemonic.ece.ucsb.edu/api.php?action=feedcontributions&feedformat=atom&user=Ryan+McGreal 2026-08-21T11:15:33Z User contributions MediaWiki 1.31.1 https://nemonic.ece.ucsb.edu/index.php?title=Products&diff=633 2023-08-30T20:47:43Z

<p>Ryan McGreal: /* Papers */</p> <hr /> <div>[[File:Nsf_par.png | center | 1296px]]<br /> <br /> All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br /> <br /> <br /> =Papers=<br /> <br /> ===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br /> http://doi.org/10.1364/AO.57.004683 <br /> <br /> Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff ( June 2018, Applied Optics)<br /> <br /> ===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br /> http://doi.org/10.1364/JOSAA.35.001438 <br /> <br /> Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. ( July 2018, Journal of the Optical Society of America A)<br /> <br /> ===Interferometric spatial frequency modulation imaging=== <br /> http://doi.org/10.1364/OL.43.005351 <br /> <br /> Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff ( October 2018, Optics Letters)<br /> <br /> ===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br /> http://doi.org/10.1364/OE.27.013015<br /> <br /> Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. ( January 2019, Optics Express)<br /> <br /> ===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br /> http://doi.org/10.1364/OL.44.002085 <br /> <br /> Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. ( April 2019, Optics Letters)<br /> <br /> ===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br /> https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br /> <br /> Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. ( May 2019, OSA Technical Digest (Optical Society of America, 2019))<br /> <br /> ===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br /> https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br /> <br /> Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al ( October 2019, The Journal of Neuroscience)<br /> <br /> ===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br /> https://doi.org/10.1016/j.cobme.2019.11.004<br /> <br /> Clough, MA ; Chen, JL ( December 2019, Current Opinion in Biomedical Engineering)<br /> <br /> ===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br /> http://doi.org/10.1364/OE.378460 <br /> <br /> Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff ( January 2020, Optics Express)<br /> <br /> ===Simultaneous multi-dimensional spatial frequency modulation imaging===<br /> https://doi.org/10.1080/15599612.2019.1694610 <br /> <br /> Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff ( January 2020, International Journal of Optomechatronics)<br /> <br /> ===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br /> https://doi.org/10.1016/j.neuron.2020.02.004<br /> <br /> Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. ( May 2020, Neuron)<br /> <br /> ===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br /> https://www.nature.com/articles/s41592-020-0835-7<br /> <br /> Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar ( May 2020, Nature Methods)<br /> <br /> ===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br /> https://doi.org/10.1038/s41467-020-17283-5<br /> <br /> Sit, Kevin K. ; Goard, Michael J. ( July 2020, Nature Communications)<br /> <br /> ===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br /> https://doi.org/10.1038/s41598-020-73438-w<br /> <br /> Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash ( October 2020, Scientific Reports)<br /> <br /> ===Single-pixel fluorescent diffraction tomography===<br /> https://doi.org/10.1364/OPTICA.400547 <br /> <br /> Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( November 2020, Optica)<br /> <br /> ===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br /> https://doi.org/10.1152/jn.00431.2020<br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( November 2020, Journal of Neurophysiology)<br /> <br /> ===Integrated dispersion compensated mode-locked quantum dot laser===<br /> https://doi.org/10.1364/PRJ.397175<br /> <br /> Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. ( January 2020, Photonics Research)<br /> <br /> ===Improving laser standards for three-photon microscopy===<br /> https://doi.org/10.1117/1.NPh.8.1.015009<br /> <br /> Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash ( January 2021, Neurophotonics)<br /> <br /> ===Stimulus-dependent representational drift in primary visual cortex===<br /> https://doi.org/10.1038/s41467-021-25436-3 <br /> <br /> Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br /> <br /> ===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br /> https://doi.org/10.1038/s41467-021-25437-2 <br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br /> <br /> ===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br /> https://doi.org/10.1126/sciadv.abf9815<br /> <br /> Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br /> https://doi.org/10.1002/adbi.202100842<br /> <br /> Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br /> <br /> ===Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report===<br /> https://doi.org/10.1117/1.NPh.9.S1.013001<br /> <br /> Abdelfattah, Ahmed ; Allu, Srinivasa Rao ; Campbell, Robert E. ; Cheng, Xiaojun ; Cižmár, Tomáš ; Costantini, Irene ; Emiliani, Valentina ; Fomin-Thunemann, Natalie ; Gilad, Ariel ; Fernández Alfonso, Tomás ; et al ( January 2022 , Neurophotonics)<br /> <br /> ===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br /> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br /> <br /> Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br /> <br /> ===Selective representations of texture and motion in mouse higher visual areas===<br /> https://doi.org/10.1016/j.cub.2022.04.091<br /> <br /> Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br /> <br /> ===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br /> https://doi.org/10.1016/j.neuroimage.2022.118978 <br /> <br /> Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br /> <br /> ===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br /> https://doi.org/10.7554/eLife.75391<br /> <br /> Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br /> <br /> ===Tomographic single pixel spatial frequency projection imaging===<br /> https://doi.org/10.1016/j.optcom.2022.128401 <br /> <br /> Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br /> <br /> ===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br /> https://doi.org/10.34133/icomputing.0003<br /> <br /> Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)<br /> <br /> ===Coregistration of heading to visual cues in retrosplenial cortex===<br /> https://doi.org/10.1038/s41467-023-37704-5<br /> <br /> Sit, Kevin K. ; Goard, Michael J. ( April 2023 , Nature Communications)<br /> <br /> ===Enhanced resolution dispersion optimized multiphoton microscopy with spatial frequency modulation imaging===<br /> https://doi.org/10.1117/12.2650490<br /> <br /> <br /> <br /> ===Design and analysis of polygonal mirror-based scan engines for improved spatial frequency modulation imaging===<br /> https://doi.org/10.1364/AO.487907<br /> <br /> Scarbrough, Daniel ; Cottrell, Seth ; Czerski, John ; Kingsolver, Ian ; Field, Jeff ; Bartels, Randy ; Squier, Jeff ( May 2023 , Applied Optics)<br /> <br /> ===Super-resolution computational saturated absorption microscopy===<br /> https://doi.org/10.1364/JOSAA.482203<br /> <br /> Murray, Gabe ; Stockton, Patrick A. ; Field, Jeff ; Pezeshki, Ali ; Squier, Jeff ; Bartels, Randy A. ( June 2023 , Journal of the Optical Society of America A)<br /> <br /> ===Optical coherence tomography-based design for a real-time motion corrected scanning microscope===<br /> https://doi.org/10.1364/OL.490087<br /> <br /> Tucker, Stephen S. ; Giblin, John T. ; Kiliç, Kivilcim ; Chen, Anderson ; Tang, Jianbo ; Boas, David A. ( July 2023 , Optics Letters)<br /> <br /> ===Single-shot spatial frequency modulation for imaging===<br /> https://doi.org/10.1364/OE.493530<br /> <br /> Cottrell, Seth ; Czerski, John ; Adams, Dan ; Field, Jeff ; Bartels, Randy ; Squier, Jeff ( July 2023 , Optics Express)</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Products&diff=632 2023-08-30T20:32:53Z

<p>Ryan McGreal: /* Papers */</p> <hr /> <div>[[File:Nsf_par.png | center | 1296px]]<br /> <br /> All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br /> <br /> <br /> =Papers=<br /> <br /> ===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br /> http://doi.org/10.1364/AO.57.004683 <br /> <br /> Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff ( June 2018, Applied Optics)<br /> <br /> ===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br /> http://doi.org/10.1364/JOSAA.35.001438 <br /> <br /> Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. ( July 2018, Journal of the Optical Society of America A)<br /> <br /> ===Interferometric spatial frequency modulation imaging=== <br /> http://doi.org/10.1364/OL.43.005351 <br /> <br /> Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff ( October 2018, Optics Letters)<br /> <br /> ===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br /> http://doi.org/10.1364/OE.27.013015<br /> <br /> Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. ( January 2019, Optics Express)<br /> <br /> ===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br /> http://doi.org/10.1364/OL.44.002085 <br /> <br /> Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. ( April 2019, Optics Letters)<br /> <br /> ===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br /> https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br /> <br /> Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. ( May 2019, OSA Technical Digest (Optical Society of America, 2019))<br /> <br /> ===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br /> https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br /> <br /> Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al ( October 2019, The Journal of Neuroscience)<br /> <br /> ===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br /> https://doi.org/10.1016/j.cobme.2019.11.004<br /> <br /> Clough, MA ; Chen, JL ( December 2019, Current Opinion in Biomedical Engineering)<br /> <br /> ===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br /> http://doi.org/10.1364/OE.378460 <br /> <br /> Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff ( January 2020, Optics Express)<br /> <br /> ===Simultaneous multi-dimensional spatial frequency modulation imaging===<br /> https://doi.org/10.1080/15599612.2019.1694610 <br /> <br /> Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff ( January 2020, International Journal of Optomechatronics)<br /> <br /> ===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br /> https://doi.org/10.1016/j.neuron.2020.02.004<br /> <br /> Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. ( May 2020, Neuron)<br /> <br /> ===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br /> https://www.nature.com/articles/s41592-020-0835-7<br /> <br /> Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar ( May 2020, Nature Methods)<br /> <br /> ===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br /> https://doi.org/10.1038/s41467-020-17283-5<br /> <br /> Sit, Kevin K. ; Goard, Michael J. ( July 2020, Nature Communications)<br /> <br /> ===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br /> https://doi.org/10.1038/s41598-020-73438-w<br /> <br /> Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash ( October 2020, Scientific Reports)<br /> <br /> ===Single-pixel fluorescent diffraction tomography===<br /> https://doi.org/10.1364/OPTICA.400547 <br /> <br /> Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( November 2020, Optica)<br /> <br /> ===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br /> https://doi.org/10.1152/jn.00431.2020<br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( November 2020, Journal of Neurophysiology)<br /> <br /> ===Integrated dispersion compensated mode-locked quantum dot laser===<br /> https://doi.org/10.1364/PRJ.397175<br /> <br /> Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. ( January 2020, Photonics Research)<br /> <br /> ===Improving laser standards for three-photon microscopy===<br /> https://doi.org/10.1117/1.NPh.8.1.015009<br /> <br /> Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash ( January 2021, Neurophotonics)<br /> <br /> ===Stimulus-dependent representational drift in primary visual cortex===<br /> https://doi.org/10.1038/s41467-021-25436-3 <br /> <br /> Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br /> <br /> ===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br /> https://doi.org/10.1038/s41467-021-25437-2 <br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br /> <br /> ===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br /> https://doi.org/10.1126/sciadv.abf9815<br /> <br /> Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br /> https://doi.org/10.1002/adbi.202100842<br /> <br /> Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br /> <br /> ===Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report===<br /> https://doi.org/10.1117/1.NPh.9.S1.013001<br /> <br /> Abdelfattah, Ahmed ; Allu, Srinivasa Rao ; Campbell, Robert E. ; Cheng, Xiaojun ; Cižmár, Tomáš ; Costantini, Irene ; Emiliani, Valentina ; Fomin-Thunemann, Natalie ; Gilad, Ariel ; Fernández Alfonso, Tomás ; et al ( January 2022 , Neurophotonics)<br /> <br /> ===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br /> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br /> <br /> Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br /> <br /> ===Selective representations of texture and motion in mouse higher visual areas===<br /> https://doi.org/10.1016/j.cub.2022.04.091<br /> <br /> Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br /> <br /> ===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br /> https://doi.org/10.1016/j.neuroimage.2022.118978 <br /> <br /> Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br /> <br /> ===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br /> https://doi.org/10.7554/eLife.75391<br /> <br /> Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br /> <br /> ===Tomographic single pixel spatial frequency projection imaging===<br /> https://doi.org/10.1016/j.optcom.2022.128401 <br /> <br /> Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br /> <br /> ===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br /> https://doi.org/10.34133/icomputing.0003<br /> <br /> Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)<br /> <br /> ===Coregistration of heading to visual cues in retrosplenial cortex===<br /> https://doi.org/10.1038/s41467-023-37704-5<br /> <br /> Sit, Kevin K. ; Goard, Michael J. ( April 2023 , Nature Communications)<br /> <br /> ===Super-resolution computational saturated absorption microscopy===<br /> https://doi.org/10.1364/JOSAA.482203<br /> <br /> Murray, Gabe ; Stockton, Patrick A. ; Field, Jeff ; Pezeshki, Ali ; Squier, Jeff ; Bartels, Randy A. ( June 2023 , Journal of the Optical Society of America A)<br /> <br /> ===Optical coherence tomography-based design for a real-time motion corrected scanning microscope===<br /> https://doi.org/10.1364/OL.490087<br /> <br /> Tucker, Stephen S. ; Giblin, John T. ; Kiliç, Kivilcim ; Chen, Anderson ; Tang, Jianbo ; Boas, David A. ( July 2023 , Optics Letters)<br /> <br /> ===Single-shot spatial frequency modulation for imaging===<br /> https://doi.org/10.1364/OE.493530<br /> <br /> Cottrell, Seth ; Czerski, John ; Adams, Dan ; Field, Jeff ; Bartels, Randy ; Squier, Jeff ( July 2023 , Optics Express)</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Products&diff=631 2023-08-30T20:00:30Z

<p>Ryan McGreal: /* Papers */</p> <hr /> <div>[[File:Nsf_par.png | center | 1296px]]<br /> <br /> All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br /> <br /> <br /> =Papers=<br /> <br /> ===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br /> http://doi.org/10.1364/AO.57.004683 <br /> <br /> Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff ( June 2018, Applied Optics)<br /> <br /> ===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br /> http://doi.org/10.1364/JOSAA.35.001438 <br /> <br /> Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. ( July 2018, Journal of the Optical Society of America A)<br /> <br /> ===Interferometric spatial frequency modulation imaging=== <br /> http://doi.org/10.1364/OL.43.005351 <br /> <br /> Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff ( October 2018, Optics Letters)<br /> <br /> ===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br /> http://doi.org/10.1364/OE.27.013015<br /> <br /> Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. ( January 2019, Optics Express)<br /> <br /> ===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br /> http://doi.org/10.1364/OL.44.002085 <br /> <br /> Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. ( April 2019, Optics Letters)<br /> <br /> ===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br /> https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br /> <br /> Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. ( May 2019, OSA Technical Digest (Optical Society of America, 2019))<br /> <br /> ===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br /> https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br /> <br /> Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al ( October 2019, The Journal of Neuroscience)<br /> <br /> ===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br /> https://doi.org/10.1016/j.cobme.2019.11.004<br /> <br /> Clough, MA ; Chen, JL ( December 2019, Current Opinion in Biomedical Engineering)<br /> <br /> ===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br /> http://doi.org/10.1364/OE.378460 <br /> <br /> Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff ( January 2020, Optics Express)<br /> <br /> ===Simultaneous multi-dimensional spatial frequency modulation imaging===<br /> https://doi.org/10.1080/15599612.2019.1694610 <br /> <br /> Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff ( January 2020, International Journal of Optomechatronics)<br /> <br /> ===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br /> https://doi.org/10.1016/j.neuron.2020.02.004<br /> <br /> Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. ( May 2020, Neuron)<br /> <br /> ===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br /> https://www.nature.com/articles/s41592-020-0835-7<br /> <br /> Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar ( May 2020, Nature Methods)<br /> <br /> ===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br /> https://doi.org/10.1038/s41467-020-17283-5<br /> <br /> Sit, Kevin K. ; Goard, Michael J. ( July 2020, Nature Communications)<br /> <br /> ===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br /> https://doi.org/10.1038/s41598-020-73438-w<br /> <br /> Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash ( October 2020, Scientific Reports)<br /> <br /> ===Single-pixel fluorescent diffraction tomography===<br /> https://doi.org/10.1364/OPTICA.400547 <br /> <br /> Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( November 2020, Optica)<br /> <br /> ===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br /> https://doi.org/10.1152/jn.00431.2020<br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( November 2020, Journal of Neurophysiology)<br /> <br /> ===Integrated dispersion compensated mode-locked quantum dot laser===<br /> https://doi.org/10.1364/PRJ.397175<br /> <br /> Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. ( January 2020, Photonics Research)<br /> <br /> ===Improving laser standards for three-photon microscopy===<br /> https://doi.org/10.1117/1.NPh.8.1.015009<br /> <br /> Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash ( January 2021, Neurophotonics)<br /> <br /> ===Stimulus-dependent representational drift in primary visual cortex===<br /> https://doi.org/10.1038/s41467-021-25436-3 <br /> <br /> Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br /> <br /> ===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br /> https://doi.org/10.1038/s41467-021-25437-2 <br /> <br /> Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br /> <br /> ===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br /> https://doi.org/10.1126/sciadv.abf9815<br /> <br /> Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br /> https://doi.org/10.1002/adbi.202100842<br /> <br /> Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br /> <br /> ===Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report===<br /> https://doi.org/10.1117/1.NPh.9.S1.013001<br /> <br /> Abdelfattah, Ahmed ; Allu, Srinivasa Rao ; Campbell, Robert E. ; Cheng, Xiaojun ; Cižmár, Tomáš ; Costantini, Irene ; Emiliani, Valentina ; Fomin-Thunemann, Natalie ; Gilad, Ariel ; Fernández Alfonso, Tomás ; et al ( January 2022 , Neurophotonics)<br /> <br /> ===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br /> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br /> <br /> Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br /> <br /> ===Selective representations of texture and motion in mouse higher visual areas===<br /> https://doi.org/10.1016/j.cub.2022.04.091<br /> <br /> Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br /> <br /> ===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br /> https://doi.org/10.1016/j.neuroimage.2022.118978 <br /> <br /> Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br /> <br /> ===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br /> https://doi.org/10.7554/eLife.75391<br /> <br /> Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br /> <br /> ===Tomographic single pixel spatial frequency projection imaging===<br /> https://doi.org/10.1016/j.optcom.2022.128401 <br /> <br /> Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br /> <br /> ===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br /> https://doi.org/10.34133/icomputing.0003<br /> <br /> Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)<br /> <br /> ===Super-resolution computational saturated absorption microscopy===<br /> https://doi.org/10.1364/JOSAA.482203<br /> <br /> Murray, Gabe ; Stockton, Patrick A. ; Field, Jeff ; Pezeshki, Ali ; Squier, Jeff ; Bartels, Randy A. ( June 2023 , Journal of the Optical Society of America A)<br /> <br /> ===Optical coherence tomography-based design for a real-time motion corrected scanning microscope===<br /> https://doi.org/10.1364/OL.490087<br /> <br /> Tucker, Stephen S. ; Giblin, John T. ; Kiliç, Kivilcim ; Chen, Anderson ; Tang, Jianbo ; Boas, David A. ( July 2023 , Optics Letters)<br /> <br /> ===Single-shot spatial frequency modulation for imaging===<br /> https://doi.org/10.1364/OE.493530<br /> <br /> Cottrell, Seth ; Czerski, John ; Adams, Dan ; Field, Jeff ; Bartels, Randy ; Squier, Jeff ( July 2023 , Optics Express)</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=630 2023-06-06T16:03:09Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will be an important, impactful project for the neuroscience community, accelerating research and supporting diverse researchers.<br /> <br /> Large field-of-view multiphoton imaging and optogenetic activation systems enable a broad array of state-of-the-art neuroscience research. However, it is expensive, both in cost and human capital. These systems cost hundreds of thousands of dollars and to make the most of them requires high level expertise. Thus, it is not as simple as going across the hall and borrowing one. These cost and expertise barriers disproportionately affect new labs as they cannot take the financial risk to purchase a system without pilot data, and/or expertise. Thus, rapid technological advancements in systems neuroscience can remain out-of-reach of diverse and creative scientists nationwide.<br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens. <br /> <br /> Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.<br /> <br /> Diesel2p is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems. <br /> <br /> We expect this important project to have a large positive impact on neuroscience research.<br /> <br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=629 2023-06-06T16:02:58Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will be an important, impactful project for the neuroscience community, accelerating research and supporting diverse researchers.<br /> <br /> Large field-of-view multiphoton imaging and optogenetic activation systems enable a broad array of state-of-the-art neuroscience research. However, it is expensive, both in cost and human capital. These systems cost hundreds of thousands of dollars and to make the most of them requires high level expertise. Thus, it is not as simple as going across the hall and borrowing one. These cost and expertise barriers disproportionately affect new labs as they cannot take the financial risk to purchase a system without pilot data, and/or expertise. Thus, rapid technological advancements in systems neuroscience can remain out-of-reach of diverse and creative scientists nationwide.<br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens. <br /> <br /> Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.<br /> <br /> This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems. <br /> <br /> We expect this important project to have a large positive impact on neuroscience research.<br /> <br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=628 2023-06-06T16:02:32Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will be an important, impactful project for the neuroscience community, accelerating research and supporting diverse researchers.<br /> <br /> Large field-of-view multiphoton imaging and optogenetic activation systems enable a broad array of state-of-the-art neuroscience research. However, it is expensive, both in cost and human capital. These systems cost hundreds of thousands of dollars and to make the most of them requires high level expertise. Thus, it is not as simple as going across the hall and borrowing one. These cost and expertise barriers disproportionately affect new labs as they cannot take the financial risk to purchase a system without pilot data, and/or expertise. Thus, rapid technological advancements in systems neuroscience can remain out-of-reach of diverse and creative scientists nationwide.<br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens. <br /> <br /> Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.<br /> <br /> In particular, we’re excited to see this being done with the Diesel2p platform. This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems. <br /> <br /> We expect this important project to have a large positive impact on neuroscience research.<br /> <br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=627 2023-06-06T14:55:22Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will be an important, impactful project for the neuroscience community, accelerating research and supporting diverse researchers.<br /> <br /> Large field-of-view multiphoton imaging and optogenetic activation systems enable a broad array of state-of-the-art neuroscience research. However, it is expensive, both in cost and human capital. These systems cost hundreds of thousands of dollars and to make the most of them requires high level expertise. Thus, it is not as simple as going across the hall and borrowing one. These cost and expertise barriers disproportionately affect new labs as they cannot take the financial risk to purchase a system without pilot data, and/or expertise. Thus, rapid technological advancements in systems neuroscience can remain out-of-reach of diverse and creative scientists nationwide.<br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens. <br /> <br /> Both models will be used to benefit the needs of diverse researchers nationwide. We are excited to see that the Smith lab is setting up such a dissemination hub. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.<br /> <br /> In particular, we’re excited to see this being done with the Diesel2p platform. This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems. <br /> <br /> We expect this important project to have a large positive impact on neuroscience research.<br /> <br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=626 2023-06-06T00:32:53Z

<p>Ryan McGreal: /* What is DieselHub? */</p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community by making several Diesel2p microscopes available for community use. <br /> <br /> Not only is multiphoton imaging equipment expensive, it requires expertise to operate that few labs have. Thus, even if imaging rigs are available at a university or institute, it is not as simple as going across the hall to use one as the expertise to use such technology is the second bottleneck, after the expense of equipment. These barriers disproportionately affect new labs as they cannot take the financial risk to purchase an imaging rig without pilot data, in addition to requiring the appropriate mentorship and training on the use of such technology. <br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB DieselHub will host a centralized hub similar to an observatory model used in astronomy. The instrumentation as well as the technical expertise will be hosted at UCSB, eliminating the barriers of both equipment expense and expertise to selected users. This model is similar to the successful model demonstrated by the OpenScope project at the Allen Institute for Brain Science.<br /> <br /> The second model involves traveling instrumentation. DieselHub will set up our imaging rigs at selected user institutes and train personnel on the use of our rigs. By bringing the microscopes to the users, we will make certain experiments more practical by eliminating the need for users to travel to UCSB as well as eliminating the need for users to ship and retrain animals. This model is similar to how microscope companies set up demonstration equipment at user facilities so that researchers can use new state-of-the-art equipment, such as the Nikon BioImaging Centers and Olympus Discovery Centers. <br /> <br /> Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.<br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=625 2023-06-06T00:29:18Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community. <br /> <br /> Not only is multiphoton imaging equipment expensive, it requires expertise to operate that few labs have. Thus, even if imaging rigs are available at a university or institute, it is not as simple as going across the hall to use one as the expertise to use such technology is the second bottleneck, after the expense of equipment. These barriers disproportionately affect new labs as they cannot take the financial risk to purchase an imaging rig without pilot data, in addition to requiring the appropriate mentorship and training on the use of such technology. <br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB DieselHub will host a centralized hub similar to an observatory model used in astronomy. The instrumentation as well as the technical expertise will be hosted at UCSB, eliminating the barriers of both equipment expense and expertise to selected users. This model is similar to the successful model demonstrated by the OpenScope project at the Allen Institute for Brain Science.<br /> <br /> The second model involves traveling instrumentation. DieselHub will set up our imaging rigs at selected user institutes and train personnel on the use of our rigs. By bringing the microscopes to the users, we will make certain experiments more practical by eliminating the need for users to travel to UCSB as well as eliminating the need for users to ship and retrain animals. This model is similar to how microscope companies set up demonstration equipment at user facilities so that researchers can use new state-of-the-art equipment, such as the Nikon BioImaging Centers and Olympus Discovery Centers. <br /> <br /> Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.<br /> <br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=624 2023-06-05T21:40:39Z

<p>Ryan McGreal: /* What is DieselHub? */</p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community. <br /> <br /> Not only is multiphoton imaging equipment expensive, it requires expertise to operate that few labs have. Thus, even if imaging rigs are available at a university or institute, it is not as simple as going across the hall to use one as the expertise to use such technology is the second bottleneck, after the expense of equipment. These barriers disproportionately affect new labs as they cannot take the financial risk to purchase an imaging rig without pilot data, in addition to requiring the appropriate mentorship and training on the use of such technology. <br /> <br /> DieselHub will overcome these barriers with two models. First, at UCSB DieselHub will host a centralized hub similar to an observatory model used in astronomy. The instrumentation as well as the technical expertise will be hosted at UCSB, eliminating the barriers of both equipment expense and expertise to selected users. This model is similar to the successful model demonstrated by the OpenScope project at the Allen Institute for Brain Science.<br /> <br /> The second model involves traveling instrumentation. DieselHub will set up our imaging rigs at selected user institutes and train personnel on the use of our rigs. By bringing the microscopes to the users, we will make certain experiments more practical by eliminating the need for users to travel to UCSB as well as eliminating the need for users to ship and retrain animals. This model is similar to how microscope companies set up demonstration equipment at user facilities so that researchers can use new state-of-the-art equipment, such as the Nikon BioImaging Centers and Olympus Discovery Centers. <br /> <br /> Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.<br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=623 2023-06-05T21:38:59Z

<p>Ryan McGreal: /* What is DieselHub? */</p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> Cost and technical expertise are two key barriers to wider adoption of next-generation multiphoton imaging systems. A commercially available large FOV two-photon imaging system costs over 600,000 USD (ThorLabs), despite still lacking multi-region two-photon optogenetic stimulation capabilities. Even once a lab procures the funds, the systems require technical expertise to take full advantage of the technology and lead to good experiments and insights. These barriers are particularly acute for new labs that cannot take a risk on such a heavy investment without pilot data and training with the technology. To overcome these barriers, it is compelling to pursue alternative dissemination strategies. We propose to make Diesel2p systems available widely across the US, at little-to-no cost, and with expert guidance.<br /> <br /> There are two existing models for technology dissemination that we can build upon. The first model is a centralized facility. Similar to observatories in the field of astronomy, the concept is to centralize the instrumentation and technical personnel, and solicit applications from external users. Recently, the Allen Institute for Brain Science launched the OpenScope project (U24NS113646). Studies are coming out from that project and neuroscience can benefit from spreading the model more broadly. <br /> <br /> A second model is traveling instrumentation. There is a long tradition of microscope companies setting up demonstration facilities at universities so that researchers can learn about and use state-of-the-art equipment. For example, the Nikon BioImaging Centers and Olympus Discovery Centers are located at universities, providing access to state-of-the-art instrumentation. This model is particularly useful when centralized experiments are not <br /> practical, such as animals that cannot be easily shipped, or require extensive training. A traveling imaging system can be shipped to where the animals are maintained, experts can provide training and support, and the imaging experiment can be performed on-site. <br /> <br /> DieselHub will use both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.<br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=622 2023-06-05T21:33:51Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> Cost and technical expertise are two key barriers to wider adoption of next-generation multiphoton imaging systems. A commercially available large FOV two-photon imaging system costs over 600,000 USD (ThorLabs), despite still lacking multi-region two-photon optogenetic stimulation capabilities. Even once a lab procures the funds, the systems require technical expertise to take full advantage of the technology and lead to good experiments and insights. These barriers are particularly acute for new labs that cannot take a risk on such a heavy investment without pilot data and training with the technology. To overcome these barriers, it is compelling to pursue alternative dissemination strategies. We propose to make Diesel2p systems available widely across the US, at little-to-no cost, and with expert guidance.<br /> <br /> There are two existing models for technology dissemination that we can build upon. The first model is a centralized facility. Similar to observatories in the field of astronomy, the concept is to centralize the instrumentation and technical personnel, and solicit applications from external users. Recently, the Allen Institute for Brain Science launched the OpenScope project (U24NS113646). Studies are coming out from that project and neuroscience can benefit from spreading the model more broadly. <br /> <br /> A second model is traveling instrumentation. There is a long tradition of microscope companies setting up demonstration facilities at universities so that researchers can learn about and use state-of-the-art equipment. For example, the Nikon BioImaging Centers and Olympus Discovery Centers are located at universities, providing access to state-of-the-art instrumentation. This model is particularly useful when centralized experiments are not <br /> practical, such as animals that cannot be easily shipped, or require extensive training. A traveling imaging system can be shipped to where the animals are maintained, experts can provide training and support, and the imaging experiment can be performed on-site. <br /> <br /> Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives. <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=621 2023-05-25T19:16:52Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> <br /> In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=620 2023-05-25T19:10:18Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=619 2023-05-25T19:09:48Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> [[File:Comparison_table_01.jpg|thumb|1200px|left|caption|Comparison between Diesel2p and other available microscopes]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=618 2023-05-25T19:09:26Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> [[File:Comparison_table_01.jpg|thumb|1000px|left|caption|Comparison between Diesel2p and other available microscopes]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=617 2023-05-25T19:09:10Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> [[File:Comparison_table_01.jpg|thumb|600px|left|caption|Comparison between Diesel2p and other available microscopes]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=616 2023-05-25T19:08:54Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> [[File:Comparison_table_01.jpg|600px|left|caption|Comparison between Diesel2p and other available microscopes]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=614 2023-05-25T18:58:45Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> ==What is DieselHub?==<br /> <br /> <br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=613 2023-05-25T18:52:44Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=612 2023-05-25T18:52:12Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> &lt;br clear=all&gt;<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> &lt;br clear=all&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=611 2023-05-25T18:51:42Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|left|caption|Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=610 2023-05-25T18:50:53Z

<p>Ryan McGreal: /* Other Diesel Systems */</p> <hr /> <div><br /> This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|caption| Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=609 2023-05-25T18:50:25Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> <br /> <br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=608 2023-05-25T18:50:08Z

<p>Ryan McGreal: </p> <hr /> <div><br /> This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br /> &lt;br clear=all&gt;<br /> ==What is Diesel2p?==<br /> <br /> Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br /> <br /> [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br /> [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==Overview of specifications==<br /> <br /> <br /> '''Diesel2p'''<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br /> <br /> -Scan angles up to ±5 degrees at the objective back aperture <br /> <br /> -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br /> <br /> <br /> ==Other Diesel Systems==<br /> <br /> '''Quadroscope'''<br /> <br /> [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -4-channel microscope (2 per scan engine)<br /> <br /> -Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br /> <br /> <br /> '''Diesel3p'''<br /> <br /> [[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]]<br /> <br /> -Dual independent scanning engines<br /> <br /> -Large field of view: ~5 x 5 mm2<br /> <br /> -Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br /> <br /> -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br /> <br /> %==Users==<br /> <br /> %&lt;gallery widths=300px heights=200px mode=&quot;nolines&quot;&gt;<br /> <br /> %Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br /> %Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br /> %Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br /> <br /> &lt;/gallery&gt;<br /> <br /> ==Publications==<br /> <br /> ===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br /> https://doi.org/10.1038/s41467-021-26736-4<br /> <br /> Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br /> <br /> ===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br /> https://doi.org/10.1038/s41467-021-26737-3<br /> <br /> Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br /> <br /> <br /> ==Lens prescriptions==<br /> <br /> [https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br /> <br /> <br /> ==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br /> <br /> https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=607 2023-02-21T00:48:46Z

<p>Ryan McGreal: </p> <hr /> <div>&lt;br clear=all&gt;<br /> <br /> The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br /> personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br /> developed by the Nemonic consortium.<br /> <br /> ==2023 Workshop==<br /> <br /> <br /> [[File:Nemonic_draft.jpg|center]]<br /> <br /> ==2022 Speakers==<br /> <br /> &lt;gallery widths=300px heights=200px mode=&quot;nolines&quot;&gt;<br /> Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br /> Image:Xin_Ye.jpg|Xin Ye, BU<br /> Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br /> Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br /> Image:Simon.jpg|Simon Schultz, Imperial College London<br /> Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br /> Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br /> Image:Kawasi.jpg|Kawasi Lett, Cornell<br /> Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br /> Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br /> Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br /> <br /> &lt;/gallery&gt;<br /> <br /> ==2022 Videos==<br /> <br /> <br /> <br /> {{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/63p3tHT6FI0|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/rUwIqU6gVvw|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/FZG1SMwf_CE|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/ZGQgpG3ByYY|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/OK-EKPIgKFQ|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br /> <br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==2022 Software Tutorials==<br /> <br /> [https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br /> <br /> [https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br /> <br /> [https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br /> <br /> [https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br /> <br /> [https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br /> <br /> [https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br /> <br /> == 2021 Speakers ==<br /> <br /> &lt;gallery widths=300px heights=200px mode=&quot;nolines&quot;&gt;<br /> Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br /> Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br /> Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br /> Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br /> Image:Kawasi.jpg|Kawasi Lett, Cornell<br /> Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br /> Image:Adam.jpg|Adam Heiniger, TOPTICA<br /> <br /> Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br /> <br /> &lt;/gallery&gt;<br /> <br /> ==2021 Videos==<br /> <br /> <br /> <br /> {{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==2021 Software Tutorials==<br /> <br /> [https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br /> <br /> [https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br /> <br /> [https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br /> <br /> [https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br /> <br /> == 2020 Speakers ==<br /> <br /> &lt;gallery widths=300px heights=200px mode=&quot;nolines&quot;&gt;<br /> Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br /> Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br /> Image:Rick.jpg|Rick Ayer, Sutter Instrument<br /> Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br /> Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br /> Image:Simon.jpg|Simon Schultz, Imperial College London<br /> Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br /> <br /> &lt;/gallery&gt;<br /> <br /> <br /> <br /> ==2020 Videos==<br /> <br /> <br /> <br /> {{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br /> <br /> <br /> {{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br /> <br /> {{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br /> <br /> &lt;br clear=all&gt;<br /> <br /> <br /> ==2020 Workshop Slideshows==<br /> <br /> [https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br /> <br /> [https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br /> <br /> [https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br /> <br /> [https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br /> <br /> [https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br /> <br /> ==2020 Related Resources==<br /> <br /> https://www.youtube.com/watch?v=DLTaqzz8hJM&amp;feature=youtu.be<br /> <br /> == 2019 Speakers ==<br /> <br /> &lt;gallery widths=300px heights=200px mode=&quot;nolines&quot;&gt;<br /> Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br /> Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br /> Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br /> Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br /> Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br /> Image:Simon.jpg|Simon Schultz, Imperial College London<br /> Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br /> <br /> &lt;/gallery&gt;<br /> <br /> &lt;br clear=all&gt;<br /> <br /> == 2019 Workshop Slideshows ==<br /> <br /> [https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br /> <br /> [https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br /> <br /> [https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br /> <br /> [https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br /> <br /> [https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br /> <br /> [https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br /> <br /> == 2019 Related Resources==<br /> <br /> [https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br /> <br /> [https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br /> <br /> [https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br /> <br /> [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br /> <br /> [https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br /> <br /> [https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br /> <br /> == See Also ==<br /> <br /> [[Big Brain]]<br /> <br /> &lt;br clear=all&gt;</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Big_Brain&diff=603 2023-01-13T00:46:36Z

<p>Ryan McGreal: </p> <hr /> <div>&lt;br clear=all&gt;<br /> <br /> Final Schedule for the Big Brain Imaging Workshop can be found '''[[Media:2022_BIG_BRAIN_WORKSHOP_final.pdf|here]]'''<br /> <br /> '''Event has concluded. Stay tuned for future events.''' <br /> <br /> Join us for the 2022 Big Brain Imaging Workshop, brought to you by the National Science Foundation Neuronex: Next Generation Multi-photon Neuro-Imaging Consortium and the Boston University Neurophotonics Center. This two-day workshop will take place on Thursday, April 7th and continue on Friday, April 8th, 2022 at the Boston University and online.<br /> <br /> The 2022 Big Brain Imaging Workshop is an opportunity to join in the discussion on optical approaches for cellular resolution imaging of neuronal populations that have been highly instrumental for uncovering the principles of neural circuit function. To date, the microscope technology development for imaging the nervous system has largely been focused on applications on a subset of model species with smaller, optically accessible brains (ex. c. elegans, zebrafish, mice). The ability to translate these technologies to species with brains that are both physically larger and less optically accessible remains a challenge. The topic of the workshop is focused on advancing cellular resolution brain imaging techniques in “larger” brains of mammalian species (ex. rats, cats, ferrets, marmosets, macaques). The goal is to bring neuroscientists and optical engineers together to discuss challenges and come up with potential solutions that will pave the way for the next generation of microscopes that can be applied to a greater number of model organisms.<br /> <br /> The following list of Distinguished Speakers will participate in the workshop:<br /> <br /> [[File:Big_Brain_2022.png|center]]<br /> <br /> <br /> == See Also ==<br /> <br /> [[Workshops]]<br /> <br /> &lt;br clear=all&gt;</div>

Ryan McGreal https://nemonic.ece.ucsb.edu/index.php?title=Big_Brain&diff=602 2023-01-13T00:46:22Z

<p>Ryan McGreal: </p> <hr /> <div>&lt;br clear=all&gt;<br /> test<br /> Final Schedule for the Big Brain Imaging Workshop can be found '''[[Media:2022_BIG_BRAIN_WORKSHOP_final.pdf|here]]'''<br /> <br /> '''Event has concluded. Stay tuned for future events.''' <br /> <br /> Join us for the 2022 Big Brain Imaging Workshop, brought to you by the National Science Foundation Neuronex: Next Generation Multi-photon Neuro-Imaging Consortium and the Boston University Neurophotonics Center. This two-day workshop will take place on Thursday, April 7th and continue on Friday, April 8th, 2022 at the Boston University and online.<br /> <br /> The 2022 Big Brain Imaging Workshop is an opportunity to join in the discussion on optical approaches for cellular resolution imaging of neuronal populations that have been highly instrumental for uncovering the principles of neural circuit function. To date, the microscope technology development for imaging the nervous system has largely been focused on applications on a subset of model species with smaller, optically accessible brains (ex. c. elegans, zebrafish, mice). The ability to translate these technologies to species with brains that are both physically larger and less optically accessible remains a challenge. The topic of the workshop is focused on advancing cellular resolution brain imaging techniques in “larger” brains of mammalian species (ex. rats, cats, ferrets, marmosets, macaques). The goal is to bring neuroscientists and optical engineers together to discuss challenges and come up with potential solutions that will pave the way for the next generation of microscopes that can be applied to a greater number of model organisms.<br /> <br /> The following list of Distinguished Speakers will participate in the workshop:<br /> <br /> [[File:Big_Brain_2022.png|center]]<br /> <br /> <br /> == See Also ==<br /> <br /> [[Workshops]]<br /> <br /> &lt;br clear=all&gt;</div>

Ryan McGreal