https://nemonic.ece.ucsb.edu/api.php?action=feedcontributions&feedformat=atom&user=Ryan.McGrealNemonic - User contributions [en]2026-08-19T07:56:00ZUser contributionsMediaWiki 1.31.1https://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=606Workshops2023-02-06T19:43:50Z<p>Ryan.McGreal: </p>
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<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
'''We have a few spots left open first come, first serve''' Applications for the Feb. 21-23, 2023 nemonic multiphoton imaging workshop can be found '''[http://slslab.org/NWA_2023.pdf here]'''<br />
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[[File:Nemonic_draft.jpg|center]]<br />
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==2022 Speakers==<br />
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<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
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</gallery><br />
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==2022 Videos==<br />
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==2022 Software Tutorials==<br />
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[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
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[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
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[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
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[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
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[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
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[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
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== 2021 Speakers ==<br />
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<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
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Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
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</gallery><br />
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==2021 Videos==<br />
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{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
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{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br />
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{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
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{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
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==2021 Software Tutorials==<br />
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[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
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[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
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[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
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== 2020 Speakers ==<br />
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<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
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</gallery><br />
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==2020 Videos==<br />
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{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
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==2020 Workshop Slideshows==<br />
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[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
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[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
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[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
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[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
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[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
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==2020 Related Resources==<br />
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https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
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== 2019 Speakers ==<br />
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<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
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</gallery><br />
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== 2019 Workshop Slideshows ==<br />
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[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
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[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
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[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
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[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
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[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
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[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
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== 2019 Related Resources==<br />
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[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
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[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
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[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
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[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
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[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
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[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
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== See Also ==<br />
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[[Big Brain]]<br />
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<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Products&diff=605Products2023-01-13T00:50:58Z<p>Ryan.McGreal: </p>
<hr />
<div>[[File:Nsf_par.png | center | 1296px]]<br />
<br />
All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br />
<br />
<br />
=Papers=<br />
<br />
===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br />
http://doi.org/10.1364/AO.57.004683 <br />
<br />
Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff ( June 2018, Applied Optics)<br />
<br />
===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br />
http://doi.org/10.1364/JOSAA.35.001438 <br />
<br />
Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. ( July 2018, Journal of the Optical Society of America A)<br />
<br />
===Interferometric spatial frequency modulation imaging=== <br />
http://doi.org/10.1364/OL.43.005351 <br />
<br />
Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff ( October 2018, Optics Letters)<br />
<br />
===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br />
http://doi.org/10.1364/OE.27.013015<br />
<br />
Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. ( January 2019, Optics Express)<br />
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===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br />
http://doi.org/10.1364/OL.44.002085 <br />
<br />
Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. ( April 2019, Optics Letters)<br />
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===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br />
https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br />
<br />
Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. ( May 2019, OSA Technical Digest (Optical Society of America, 2019))<br />
<br />
===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br />
https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br />
<br />
Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al ( October 2019, The Journal of Neuroscience)<br />
<br />
===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br />
https://doi.org/10.1016/j.cobme.2019.11.004<br />
<br />
Clough, MA ; Chen, JL ( December 2019, Current Opinion in Biomedical Engineering)<br />
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===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br />
http://doi.org/10.1364/OE.378460 <br />
<br />
Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff ( January 2020, Optics Express)<br />
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===Simultaneous multi-dimensional spatial frequency modulation imaging===<br />
https://doi.org/10.1080/15599612.2019.1694610 <br />
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Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff ( January 2020, International Journal of Optomechatronics)<br />
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===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br />
https://doi.org/10.1016/j.neuron.2020.02.004<br />
<br />
Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. ( May 2020, Neuron)<br />
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===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br />
https://www.nature.com/articles/s41592-020-0835-7<br />
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Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar ( May 2020, Nature Methods)<br />
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===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br />
https://doi.org/10.1038/s41467-020-17283-5<br />
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Sit, Kevin K. ; Goard, Michael J. ( July 2020, Nature Communications)<br />
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===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br />
https://doi.org/10.1038/s41598-020-73438-w<br />
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Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash ( October 2020, Scientific Reports)<br />
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===Single-pixel fluorescent diffraction tomography===<br />
https://doi.org/10.1364/OPTICA.400547 <br />
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Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( November 2020, Optica)<br />
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===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br />
https://doi.org/10.1152/jn.00431.2020<br />
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Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( November 2020, Journal of Neurophysiology)<br />
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===Integrated dispersion compensated mode-locked quantum dot laser===<br />
https://doi.org/10.1364/PRJ.397175<br />
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Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. ( January 2020, Photonics Research)<br />
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===Improving laser standards for three-photon microscopy===<br />
https://doi.org/10.1117/1.NPh.8.1.015009<br />
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Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash ( January 2021, Neurophotonics)<br />
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===Stimulus-dependent representational drift in primary visual cortex===<br />
https://doi.org/10.1038/s41467-021-25436-3 <br />
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Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br />
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===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br />
https://doi.org/10.1038/s41467-021-25437-2 <br />
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Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br />
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===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br />
https://doi.org/10.1126/sciadv.abf9815<br />
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Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br />
https://doi.org/10.1002/adbi.202100842<br />
<br />
Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br />
<br />
===Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report===<br />
https://doi.org/10.1117/1.NPh.9.S1.013001<br />
<br />
Abdelfattah, Ahmed ; Allu, Srinivasa Rao ; Campbell, Robert E. ; Cheng, Xiaojun ; Cižmár, Tomáš ; Costantini, Irene ; Emiliani, Valentina ; Fomin-Thunemann, Natalie ; Gilad, Ariel ; Fernández Alfonso, Tomás ; et al ( January 2022 , Neurophotonics)<br />
<br />
===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br />
<br />
Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br />
<br />
===Selective representations of texture and motion in mouse higher visual areas===<br />
https://doi.org/10.1016/j.cub.2022.04.091<br />
<br />
Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br />
<br />
===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br />
https://doi.org/10.1016/j.neuroimage.2022.118978 <br />
<br />
Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br />
<br />
===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br />
https://doi.org/10.7554/eLife.75391<br />
<br />
Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br />
<br />
===Tomographic single pixel spatial frequency projection imaging===<br />
https://doi.org/10.1016/j.optcom.2022.128401 <br />
<br />
Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br />
<br />
===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br />
https://doi.org/10.34133/icomputing.0003<br />
<br />
Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Products&diff=604Products2023-01-13T00:50:07Z<p>Ryan.McGreal: </p>
<hr />
<div>[[File:Nsf_par.png | center | 1296px]]<br />
<br />
All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br />
<br />
<br />
=Papers=<br />
<br />
===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br />
http://doi.org/10.1364/AO.57.004683 <br />
<br />
Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff ( June 2018, Applied Optics)<br />
<br />
===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br />
http://doi.org/10.1364/JOSAA.35.001438 <br />
<br />
Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. ( July 2018, Journal of the Optical Society of America A)<br />
<br />
===Interferometric spatial frequency modulation imaging=== <br />
http://doi.org/10.1364/OL.43.005351 <br />
<br />
Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff ( October 2018, Optics Letters)<br />
<br />
===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br />
http://doi.org/10.1364/OE.27.013015<br />
<br />
Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. ( January 2019, Optics Express)<br />
<br />
===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br />
http://doi.org/10.1364/OL.44.002085 <br />
<br />
Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. ( April 2019, Optics Letters)<br />
<br />
===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br />
https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br />
<br />
Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. ( May 2019, OSA Technical Digest (Optical Society of America, 2019))<br />
<br />
===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br />
https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br />
<br />
Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al ( October 2019, The Journal of Neuroscience)<br />
<br />
===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br />
https://doi.org/10.1016/j.cobme.2019.11.004<br />
<br />
Clough, MA ; Chen, JL ( December 2019, Current Opinion in Biomedical Engineering)<br />
<br />
===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br />
http://doi.org/10.1364/OE.378460 <br />
<br />
Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff ( January 2020, Optics Express)<br />
<br />
===Simultaneous multi-dimensional spatial frequency modulation imaging===<br />
https://doi.org/10.1080/15599612.2019.1694610 <br />
<br />
Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff ( January 2020, International Journal of Optomechatronics)<br />
<br />
===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br />
https://doi.org/10.1016/j.neuron.2020.02.004<br />
<br />
Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. ( May 2020, Neuron)<br />
<br />
===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br />
https://www.nature.com/articles/s41592-020-0835-7<br />
<br />
Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar ( May 2020, Nature Methods)<br />
<br />
===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br />
https://doi.org/10.1038/s41467-020-17283-5<br />
<br />
Sit, Kevin K. ; Goard, Michael J. ( July 2020, Nature Communications)<br />
<br />
===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br />
https://doi.org/10.1038/s41598-020-73438-w<br />
<br />
Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash ( October 2020, Scientific Reports)<br />
<br />
===Single-pixel fluorescent diffraction tomography===<br />
https://doi.org/10.1364/OPTICA.400547 <br />
<br />
Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( November 2020, Optica)<br />
<br />
===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br />
https://doi.org/10.1152/jn.00431.2020<br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( November 2020, Journal of Neurophysiology)<br />
<br />
===Integrated dispersion compensated mode-locked quantum dot laser===<br />
https://doi.org/10.1364/PRJ.397175<br />
<br />
Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. ( January 2020, Photonics Research)<br />
<br />
===Improving laser standards for three-photon microscopy===<br />
https://doi.org/10.1117/1.NPh.8.1.015009<br />
<br />
Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash ( January 2021, Neurophotonics)<br />
<br />
===Stimulus-dependent representational drift in primary visual cortex===<br />
https://doi.org/10.1038/s41467-021-25436-3 <br />
<br />
Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br />
<br />
===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br />
https://doi.org/10.1038/s41467-021-25437-2 <br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br />
<br />
===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br />
https://doi.org/10.1126/sciadv.abf9815<br />
<br />
Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br />
https://doi.org/10.1002/adbi.202100842<br />
<br />
Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br />
<br />
===Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report===<br />
https://doi.org/10.1117/1.NPh.9.S1.013001<br />
<br />
Abdelfattah, Ahmed; Allu, Srinivasa Rao; Campbell, Robert E.; Cheng, Xiaojun; Cižmár, Tomáš; Costantini, Irene; Emiliani, Valentina; Fomin-Thunemann, Natalie; Gilad, Ariel; Fernández Alfonso, Tomás; Ferri, Christopher G.; Harris, Andrew; Hillman, Elizabeth M.; Holt, Matthew G.; Kiliç, Kivilcim; Miller, Evan W.; Mesquita, Rickson C.; Nadella, K.M. Naga; Nägerl, U. Valentin; Perez Campos, Citlali ; Puppo, Francesca; Shoham, Shy; Silver, R. Angus; Srinivasan, Vivek J.; Thunemann, Martin; Tian, Lei; Vinogradov, Sergei A.; Vitale, Flavia; Uhlirova, Hana; Xu, Chris; Yang, Mu-Han; Zhao, Yongxin; Ahuja, Sapna; Akkin, Taner; Brake, Joshua; Boas, David A.; Buckley, Erin M.; Chen, Anderson I.; De Vittorio, Massimo; Devor, Anna; Doran, Patrick; El Khatib, Mirna; Fainman, Yeshaiahu; Han, Xue; Hochgeschwender, Ute; Ji, Na; Lake, Evelyn; Li, Lei; Li, Tianqi; Machler, Philipp; Nasu, Yusuke; Nimmerjahn, Axel; Ondrácková, Petra; Pavone, Francesco S.; Peterka, Darcy; Pisano, Filippo; Pisanello, Ferruccio; Sabatini, Bernardo L.; Sadegh, Sanaz; Sakadžic, Sava; Shroff, Sanaya N.; Sims, Ruth R.; Smith, Spencer LaVere; Tian, Lin; Troxler, Thomas; Valera, Antoine; Vaziri, Alipasha; Wang, Lihong V.; Yang, Changhuei; Yellen, Gary; Yizhar, Ofer ( January 2022 , Neurophotonics)<br />
<br />
===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br />
<br />
Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br />
<br />
===Selective representations of texture and motion in mouse higher visual areas===<br />
https://doi.org/10.1016/j.cub.2022.04.091<br />
<br />
Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br />
<br />
===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br />
https://doi.org/10.1016/j.neuroimage.2022.118978 <br />
<br />
Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br />
<br />
===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br />
https://doi.org/10.7554/eLife.75391<br />
<br />
Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br />
<br />
===Tomographic single pixel spatial frequency projection imaging===<br />
https://doi.org/10.1016/j.optcom.2022.128401 <br />
<br />
Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br />
<br />
===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br />
https://doi.org/10.34133/icomputing.0003<br />
<br />
Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Products&diff=601Products2023-01-10T21:31:43Z<p>Ryan.McGreal: </p>
<hr />
<div>[[File:Nsf_par.png | center | 1296px]]<br />
<br />
All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br />
<br />
<br />
=Papers=<br />
<br />
===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br />
http://doi.org/10.1364/AO.57.004683 <br />
<br />
Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff (June 2018, Applied Optics)<br />
<br />
===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br />
http://doi.org/10.1364/JOSAA.35.001438 <br />
<br />
Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. (July 2018, Journal of the Optical Society of America A)<br />
<br />
===Interferometric spatial frequency modulation imaging=== <br />
http://doi.org/10.1364/OL.43.005351 <br />
<br />
Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff (October 2018, Optics Letters)<br />
<br />
===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br />
http://doi.org/10.1364/OE.27.013015<br />
<br />
Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. (January 2019, Optics Express)<br />
<br />
===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br />
http://doi.org/10.1364/OL.44.002085 <br />
<br />
Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. (April 2019, Optics Letters)<br />
<br />
===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br />
https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br />
<br />
Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. (May 2019, OSA Technical Digest (Optical Society of America, 2019))<br />
<br />
===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br />
https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br />
<br />
Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al (October 2019, The Journal of Neuroscience)<br />
<br />
===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br />
https://doi.org/10.1016/j.cobme.2019.11.004<br />
<br />
Clough, MA ; Chen, JL (December 2019, Current Opinion in Biomedical Engineering)<br />
<br />
===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br />
http://doi.org/10.1364/OE.378460 <br />
<br />
Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff (January 2020, Optics Express)<br />
<br />
===Simultaneous multi-dimensional spatial frequency modulation imaging===<br />
https://doi.org/10.1080/15599612.2019.1694610 <br />
<br />
Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff (January 2020, International Journal of Optomechatronics)<br />
<br />
===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br />
https://doi.org/10.1016/j.neuron.2020.02.004<br />
<br />
Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. (May 2020, Neuron)<br />
<br />
===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br />
https://www.nature.com/articles/s41592-020-0835-7<br />
<br />
Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar (May 2020, Nature Methods)<br />
<br />
===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br />
https://doi.org/10.1038/s41467-020-17283-5<br />
<br />
Sit, Kevin K. ; Goard, Michael J. (July 2020, Nature Communications)<br />
<br />
===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br />
https://doi.org/10.1038/s41598-020-73438-w<br />
<br />
Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash (October 2020, Scientific Reports)<br />
<br />
===Single-pixel fluorescent diffraction tomography===<br />
https://doi.org/10.1364/OPTICA.400547 <br />
<br />
Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. (November 2020, Optica)<br />
<br />
===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br />
https://doi.org/10.1152/jn.00431.2020<br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf (November 2020, Journal of Neurophysiology)<br />
<br />
===Integrated dispersion compensated mode-locked quantum dot laser===<br />
https://doi.org/10.1364/PRJ.397175<br />
<br />
Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. (January 2020, Photonics Research)<br />
<br />
===Improving laser standards for three-photon microscopy===<br />
https://doi.org/10.1117/1.NPh.8.1.015009<br />
<br />
Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash (January 2021, Neurophotonics)<br />
<br />
===Stimulus-dependent representational drift in primary visual cortex===<br />
https://doi.org/10.1038/s41467-021-25436-3 <br />
<br />
Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br />
<br />
===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br />
https://doi.org/10.1038/s41467-021-25437-2 <br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br />
<br />
===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br />
https://doi.org/10.1126/sciadv.abf9815<br />
<br />
Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br />
https://doi.org/10.1002/adbi.202100842<br />
<br />
Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br />
<br />
===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br />
<br />
Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br />
<br />
===Selective representations of texture and motion in mouse higher visual areas===<br />
https://doi.org/10.1016/j.cub.2022.04.091<br />
<br />
Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br />
<br />
===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br />
https://doi.org/10.1016/j.neuroimage.2022.118978 <br />
<br />
Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br />
<br />
===Long-term transverse imaging of the hippocampus with glass microperiscopes===<br />
https://doi.org/10.7554/eLife.75391<br />
<br />
Redman, William T ; Wolcott, Nora S ; Montelisciani, Luca ; Luna, Gabriel ; Marks, Tyler D ; Sit, Kevin K ; Yu, Che-Hang ; Smith, Spencer ; Goard, Michael J. ( July 2022 , eLife)<br />
<br />
===Tomographic single pixel spatial frequency projection imaging===<br />
https://doi.org/10.1016/j.optcom.2022.128401 <br />
<br />
Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br />
<br />
===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br />
https://doi.org/10.34133/icomputing.0003<br />
<br />
Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Products&diff=600Products2023-01-05T20:26:05Z<p>Ryan.McGreal: </p>
<hr />
<div>[[File:Nsf_par.png | center | 1296px]]<br />
<br />
All products are available on the NSF Public Access Repository (NSF-PAR) https://par.nsf.gov/<br />
<br />
<br />
=Papers=<br />
<br />
===Fabrication and characterization of modulation masks for multimodal spatial frequency modulated microscopy=== <br />
http://doi.org/10.1364/AO.57.004683 <br />
<br />
Worts, Nathan ; Young, Michael ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Squier, Jeff (June 2018, Applied Optics)<br />
<br />
===Three-dimensional single-pixel imaging of incoherent light with spatiotemporally modulated illumination=== <br />
http://doi.org/10.1364/JOSAA.35.001438 <br />
<br />
Field, Jeffrey J. ; Wernsing, Keith A. ; Squier, Jeff A. ; Bartels, Randy A. (July 2018, Journal of the Optical Society of America A)<br />
<br />
===Interferometric spatial frequency modulation imaging=== <br />
http://doi.org/10.1364/OL.43.005351 <br />
<br />
Worts, Nathan ; Field, Jeff ; Bartels, Randy ; Jones, Jason ; Broderick, Jeff ; Squier, Jeff (October 2018, Optics Letters)<br />
<br />
===Fluorescent coherent diffractive imaging with accelerating light sheets=== <br />
http://doi.org/10.1364/OE.27.013015<br />
<br />
Field, Jeffrey J. ; Squier, Jeff A. ; Bartels, Randy A. (January 2019, Optics Express)<br />
<br />
===Spectral phase and amplitude retrieval and compensation technique for measurement of pulses=== <br />
http://doi.org/10.1364/OL.44.002085 <br />
<br />
Allende Motz, Alyssa M. ; Squier, Jeff A. ; Durfee, Charles G. ; Adams, Daniel E. (April 2019, Optics Letters)<br />
<br />
===Spectral Phase and Amplitude Retrieval and Compensation for Random Access Microscopy===<br />
https://doi.org/10.1364/CLEO_AT.2019.AM2I.4<br />
<br />
Allende Motz, Alyssa M. ; Durfee, Charles G. ; Squier, Jeff A. ; Adams, Daniel E. (May 2019, OSA Technical Digest (Optical Society of America, 2019))<br />
<br />
===Advanced Circuit and Cellular Imaging Methods in Nonhuman Primates===<br />
https://doi.org/10.1523/JNEUROSCI.1168-19.2019 <br />
<br />
Macknik, Stephen L. ; Alexander, Robert G. ; Caballero, Olivya ; Chanovas, Jordi ; Nielsen, Kristina J. ; Nishimura, Nozomi ; Schaffer, Chris B. ; Slovin, Hamutal ; Babayoff, Amit ; Barak, Ravid ; et al (October 2019, The Journal of Neuroscience)<br />
<br />
===Cellular resolution imaging of neuronal activity across space and time in the mammalian brain===<br />
https://doi.org/10.1016/j.cobme.2019.11.004<br />
<br />
Clough, MA ; Chen, JL (December 2019, Current Opinion in Biomedical Engineering)<br />
<br />
===Two-dimensional random access multiphoton spatial frequency modulated imaging===<br />
http://doi.org/10.1364/OE.378460 <br />
<br />
Allende Motz, Alyssa M. ; Czerski, John ; Adams, Daniel E. ; Durfee, Charles ; Bartels, Randy ; Field, Jeff ; Hoy, Christopher L. ; Squier, Jeff (January 2020, Optics Express)<br />
<br />
===Simultaneous multi-dimensional spatial frequency modulation imaging===<br />
https://doi.org/10.1080/15599612.2019.1694610 <br />
<br />
Worts, Nathan ; Czerski, John ; Jones, Jason ; Field, Jeffrey J. ; Bartels, Randy ; Squier, Jeff (January 2020, International Journal of Optomechatronics)<br />
<br />
===Context-Dependent Sensory Processing across Primary and Secondary Somatosensory Cortex===<br />
https://doi.org/10.1016/j.neuron.2020.02.004<br />
<br />
Condylis, Cameron ; Lowet, Eric ; Ni, Jianguang ; Bistrong, Karina ; Ouellette, Timothy ; Josephs, Nathaniel ; Chen, Jerry L. (May 2020, Neuron)<br />
<br />
===jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths===<br />
https://www.nature.com/articles/s41592-020-0835-7<br />
<br />
Mohr, Manuel Alexander ; Bushey, Daniel ; Aggarwal, Abhi ; Marvin, Jonathan S. ; Kim, Jeong Jun ; Marquez, Emiliano Jimenez ; Liang, Yajie ; Patel, Ronak ; Macklin, John J. ; Lee, Chi-Yu ; Tsang, Arthur ; Tsegaye, Getahun ; Ahrens, Allison M. ; Chen, Jerry L. ; Kim, Douglas S. ; Wong, Allan M. ; Looger, Loren L. ; Schreiter, Eric R. ; Podgorski, Kaspar (May 2020, Nature Methods)<br />
<br />
===Distributed and retinotopically asymmetric processing of coherent motion in mouse visual cortex===<br />
https://doi.org/10.1038/s41467-020-17283-5<br />
<br />
Sit, Kevin K. ; Goard, Michael J. (July 2020, Nature Communications)<br />
<br />
===Three-photon imaging of synthetic dyes in deep layers of the neocortex===<br />
https://doi.org/10.1038/s41598-020-73438-w<br />
<br />
Liu, Chao J. ; Roy, Arani ; Simons, Anthony A. ; Farinella, Deano M. ; Kara, Prakash (October 2020, Scientific Reports)<br />
<br />
===Single-pixel fluorescent diffraction tomography===<br />
https://doi.org/10.1364/OPTICA.400547 <br />
<br />
Stockton, Patrick A. ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. (November 2020, Optica)<br />
<br />
===Diverse coactive neurons encode stimulus-driven and stimulus-independent variables===<br />
https://doi.org/10.1152/jn.00431.2020<br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf (November 2020, Journal of Neurophysiology)<br />
<br />
===Integrated dispersion compensated mode-locked quantum dot laser===<br />
https://doi.org/10.1364/PRJ.397175<br />
<br />
Zhang, Zeyu ; Norman, Justin C. ; Liu, Songtao ; Malik, Aditya ; Bowers, John E. (January 2020, Photonics Research)<br />
<br />
===Improving laser standards for three-photon microscopy===<br />
https://doi.org/10.1117/1.NPh.8.1.015009<br />
<br />
Farinella, Deano M. ; Roy, Arani ; Liu, Chao J. ; Kara, Prakash (January 2021, Neurophotonics)<br />
<br />
===Stimulus-dependent representational drift in primary visual cortex===<br />
https://doi.org/10.1038/s41467-021-25436-3 <br />
<br />
Marks, Tyler D. ; Goard, Michael J. ( August 2021 , Nature Communications)<br />
<br />
===Stable representation of a naturalistic movie emerges from episodic activity with gain variability===<br />
https://doi.org/10.1038/s41467-021-25437-2 <br />
<br />
Xia, Ji ; Marks, Tyler D. ; Goard, Michael J. ; Wessel, Ralf ( August 2021 , Nature Communications)<br />
<br />
===A Distributed Circuit for Associating Environmental Context to Motor Choice in Retrosplenial Cortex===<br />
https://doi.org/10.1126/sciadv.abf9815<br />
<br />
Franco, Luis M. ; Goard, Michael J. ( August 2021 , Science Advances)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals===<br />
https://doi.org/10.1002/adbi.202100842<br />
<br />
Srinivasan, Prasanna ; Griffin, Nicole M. ; Joshi, Pradeep ; Thakur, Dhananjay ; Nguyen-Le, Alex; McCotter, Sean ; Jain, Akshar; Saeidi, Mitra ; Kulkarni, Prajakta; Eisdorfer, Jaclyn T. ; Rothman, Joel ; Montell, Craig; Theogarajan, Luke ( December 2021 , Advanced Biology)<br />
<br />
===Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration===<br />
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8932665/<br />
<br />
Telias, M.+ ; Sit, KK.+ ; Smith, B. ; Misra, A. ; Goard, MJ.* ; Kramer, RH.* ( March 2022 , Science advances)<br />
<br />
===Selective representations of texture and motion in mouse higher visual areas===<br />
https://doi.org/10.1016/j.cub.2022.04.091<br />
<br />
Yu, Yiyi; Stirman, Jeffrey N.; Dorsett, Christopher R.; Smith, Spencer L. ( May 2022, Current Biology)<br />
<br />
===Cortical layer-specific differences in stimulus selectivity revealed with high-field fMRI and single-vessel resolution optical imaging of the primary visual cortex===<br />
https://doi.org/10.1016/j.neuroimage.2022.118978 <br />
<br />
Cho, Shinho ; Roy, Arani ; Liu, Chao J. ; Idiyatullin, Djaudat ; Zhu, Wei ; Zhang, Yi ; Zhu, Xiao-Hong ; O'Herron, Phillip ; Leikvoll, Austin ; Chen, Wei ; et al ( May 2022 , NeuroImage)<br />
<br />
===Tomographic single pixel spatial frequency projection imaging===<br />
https://doi.org/10.1016/j.optcom.2022.128401 <br />
<br />
Stockton, Patrick ; Murray, Gabe ; Field, Jeffrey J. ; Squier, Jeff ; Pezeshki, Ali ; Bartels, Randy A. ( October 2022 , Optics Communications)<br />
<br />
===Super-Resolution Imaging by Computationally Fusing Quantum and Classical Optical Information===<br />
https://doi.org/10.34133/icomputing.0003<br />
<br />
Bartels, Randy A. ; Murray, Gabe ; Field, Jeff ; Squier, Jeff ( January 2023 , Intelligent Computing)</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=599Workshops2022-12-06T22:12:23Z<p>Ryan.McGreal: /* 2023 Workshop */</p>
<hr />
<div><br clear=all><br />
<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
Applications for the Feb. 21-23, 2023 nemonic multiphoton imaging workshop can be found '''[http://slslab.org/NWA_2023.pdf here]'''<br />
<br />
<br />
[[File:Nemonic_draft.jpg|center]]<br />
<br />
==2022 Speakers==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2022 Videos==<br />
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{{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br />
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{{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br />
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{{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br />
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{{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br />
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<br />
<br clear=all><br />
<br />
<br />
==2022 Software Tutorials==<br />
<br />
[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
<br />
[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
<br />
[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
<br />
== 2021 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2021 Videos==<br />
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<br />
{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
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{{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br />
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{{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br />
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{{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br />
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{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
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{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2021 Software Tutorials==<br />
<br />
[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
<br />
== 2020 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br />
<br />
==2020 Videos==<br />
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{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br />
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{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
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{{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br />
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{{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br />
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{{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br />
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<br clear=all><br />
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<br />
==2020 Workshop Slideshows==<br />
<br />
[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
<br />
[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
<br />
[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
<br />
[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
<br />
==2020 Related Resources==<br />
<br />
https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
<br />
== 2019 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br clear=all><br />
<br />
== 2019 Workshop Slideshows ==<br />
<br />
[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
<br />
[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
<br />
[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
<br />
[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
<br />
[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
<br />
== 2019 Related Resources==<br />
<br />
[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
<br />
[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
<br />
[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
<br />
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
<br />
[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
<br />
[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
<br />
== See Also ==<br />
<br />
[[Big Brain]]<br />
<br />
<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=598Workshops2022-12-06T22:12:15Z<p>Ryan.McGreal: </p>
<hr />
<div><br clear=all><br />
<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
Application for the Feb. 21-23, 2023 nemonic multiphoton imaging workshop can be found '''[http://slslab.org/NWA_2023.pdf here]'''<br />
<br />
<br />
[[File:Nemonic_draft.jpg|center]]<br />
<br />
<br />
==2022 Speakers==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2022 Videos==<br />
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{{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/OK-EKPIgKFQ|640|right}}<br />
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{{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br />
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{{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br />
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{{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br />
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{{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br />
<br />
<br />
<br clear=all><br />
<br />
<br />
==2022 Software Tutorials==<br />
<br />
[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
<br />
[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
<br />
[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
<br />
== 2021 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2021 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
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{{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br />
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{{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br />
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{{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br />
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{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br />
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{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
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{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2021 Software Tutorials==<br />
<br />
[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
<br />
== 2020 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br />
<br />
==2020 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2020 Workshop Slideshows==<br />
<br />
[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
<br />
[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
<br />
[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
<br />
[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
<br />
==2020 Related Resources==<br />
<br />
https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
<br />
== 2019 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br clear=all><br />
<br />
== 2019 Workshop Slideshows ==<br />
<br />
[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
<br />
[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
<br />
[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
<br />
[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
<br />
[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
<br />
== 2019 Related Resources==<br />
<br />
[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
<br />
[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
<br />
[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
<br />
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
<br />
[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
<br />
[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
<br />
== See Also ==<br />
<br />
[[Big Brain]]<br />
<br />
<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=597Workshops2022-12-06T22:11:33Z<p>Ryan.McGreal: </p>
<hr />
<div><br clear=all><br />
<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
Application for the Feb. 21-23, 2023 nemonic multiphoton imaging workshop can be found '''[[http://slslab.org/NWA_2023.pdf|here]]'''<br />
<br />
<br />
[[File:Nemonic_draft.jpg|center]]<br />
<br />
<br />
==2022 Speakers==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2022 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/63p3tHT6FI0|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/rUwIqU6gVvw|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/FZG1SMwf_CE|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/ZGQgpG3ByYY|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/OK-EKPIgKFQ|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br />
<br />
<br />
<br clear=all><br />
<br />
<br />
==2022 Software Tutorials==<br />
<br />
[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
<br />
[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
<br />
[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
<br />
== 2021 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2021 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2021 Software Tutorials==<br />
<br />
[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
<br />
== 2020 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br />
<br />
==2020 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2020 Workshop Slideshows==<br />
<br />
[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
<br />
[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
<br />
[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
<br />
[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
<br />
==2020 Related Resources==<br />
<br />
https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
<br />
== 2019 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br clear=all><br />
<br />
== 2019 Workshop Slideshows ==<br />
<br />
[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
<br />
[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
<br />
[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
<br />
[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
<br />
[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
<br />
== 2019 Related Resources==<br />
<br />
[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
<br />
[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
<br />
[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
<br />
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
<br />
[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
<br />
[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
<br />
== See Also ==<br />
<br />
[[Big Brain]]<br />
<br />
<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=596Workshops2022-12-06T22:11:16Z<p>Ryan.McGreal: </p>
<hr />
<div><br clear=all><br />
<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
Application for the Feb. 21-23, 2023 nemonic multiphoton imaging workshop can be found '''[[Media:http://slslab.org/NWA_2023.pdf|here]]'''<br />
<br />
<br />
[[File:Nemonic_draft.jpg|center]]<br />
<br />
<br />
==2022 Speakers==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2022 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/63p3tHT6FI0|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/rUwIqU6gVvw|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/FZG1SMwf_CE|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/ZGQgpG3ByYY|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/OK-EKPIgKFQ|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br />
<br />
<br />
<br clear=all><br />
<br />
<br />
==2022 Software Tutorials==<br />
<br />
[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
<br />
[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
<br />
[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
<br />
== 2021 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2021 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2021 Software Tutorials==<br />
<br />
[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
<br />
== 2020 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br />
<br />
==2020 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br />
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{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
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{{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2020 Workshop Slideshows==<br />
<br />
[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
<br />
[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
<br />
[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
<br />
[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
<br />
==2020 Related Resources==<br />
<br />
https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
<br />
== 2019 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br clear=all><br />
<br />
== 2019 Workshop Slideshows ==<br />
<br />
[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
<br />
[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
<br />
[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
<br />
[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
<br />
[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
<br />
== 2019 Related Resources==<br />
<br />
[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
<br />
[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
<br />
[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
<br />
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
<br />
[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
<br />
[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
<br />
== See Also ==<br />
<br />
[[Big Brain]]<br />
<br />
<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Workshops&diff=595Workshops2022-12-06T22:10:02Z<p>Ryan.McGreal: </p>
<hr />
<div><br clear=all><br />
<br />
The Nemonic project holds a yearly workshops at UC Santa Barbara to help train<br />
personnel from other institutions on how to create and use novel multiphoton neuroimaging instrumentation<br />
developed by the Nemonic consortium.<br />
<br />
==2023 Workshop==<br />
<br />
[[File:Nemonic_draft.jpg|center]]<br />
<br />
<br />
==2022 Speakers==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jerome_Lecoq.jpg|Jérôme Lecoq, Allen Institute<br />
Image:Xin_Ye.jpg|Xin Ye, BU<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Allen Institute<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Bruno_Pichler.jpg|Bruno Pichler, INSS<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2022 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/tHBeF2VNfL0|640|left}}<br />
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{{#ev:youtube|https://youtu.be/63p3tHT6FI0|640|right}}<br />
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{{#ev:youtube|https://youtu.be/rUwIqU6gVvw|640|left}}<br />
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{{#ev:youtube|https://youtu.be/FZG1SMwf_CE|640|right}}<br />
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<br />
{{#ev:youtube|https://youtu.be/ZGQgpG3ByYY|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/OK-EKPIgKFQ|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/eAwGQOiSOvE|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/aZfpbt-31jw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/CJfCEegMmeQ|640|left}}<br />
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{{#ev:youtube|https://youtu.be/72sT44Y4XZM|640|right}}<br />
<br />
<br />
<br clear=all><br />
<br />
<br />
==2022 Software Tutorials==<br />
<br />
[https://github.com/AllenInstitute/deepinterpolation/blob/master/examples/GoogleColab_example_Ophys__Finetuning_and_Inference_Workshop.ipynb Ophys Finetuning and Inference - Jérôme Lecoq]<br />
<br />
[https://github.com/UNC-optics/Introduction-to-holography Introduction to Holography - Nico Pégard]<br />
<br />
[https://github.com/UNC-optics/DeepCGH Deep CGH GitHub - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1s9zPDb6TArwB4FSUBiUBIuVr9SdtrXnM?usp=sharing DeepCGH: 3D computer generated holography using deep learning - Nico Pégard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://github.com/flatironinstitute/CaImAn CaImAn GitHub - Andrea Giovannucci]<br />
<br />
== 2021 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Kawasi.jpg|Kawasi Lett, Cornell<br />
Image:Bruce.jpg|Bruce Kimmel, Vidrio Technologies<br />
Image:Adam.jpg|Adam Heiniger, TOPTICA<br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
==2021 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/840N69iuL2g|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/rFyFsfgNa3Q|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/NZZ6_zo0YIM|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/z6TlH28MLRo|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/J1z4MIerAZ0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/SKou5Watip4|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/4UHRRKJhp_Y|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/tiCPBUMHFoE|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2021 Software Tutorials==<br />
<br />
[https://github.com/UNC-optics/DeepCGH DeepCGH: 3D computer generated holography using deep learning - Nico Pegard]<br />
<br />
[https://colab.research.google.com/drive/1vkp-uPV8tKavmX12bcN2L-jYH8_MgmHL?usp=sharing CaImAn’s Demo pipeline - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1m3ZZMqb2OMpRGXRStqqfmDxDRjlHevZT?usp=sharing Complete pipeline for online processing using CaImAn Online (OnACID) - Andrea Giovannucci]<br />
<br />
[https://colab.research.google.com/drive/1F-GSVGXnL4MvoU7UyNAVTNeCAopugmwX?usp=sharing Demo pipeline for processing voltage imaging data - Andrea Giovannucci]<br />
<br />
== 2020 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Jeff_Squier_CSM.jpg|Jeff Squier, Colorado School of Mines<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Rick.jpg|Rick Ayer, Sutter Instrument<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br />
<br />
==2020 Videos==<br />
<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/KjHrjhvhRy0|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/JAA89HQRaLw|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/AT6cIu-hmcc|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/S8wy6dARktk|640|right}}<br />
<br />
<br />
{{#ev:youtube|https://youtu.be/UNSTEdjJZEU|640|left}}<br />
<br />
{{#ev:youtube|https://youtu.be/GImZdKUwvuc|640|right}}<br />
<br />
<br clear=all><br />
<br />
<br />
==2020 Workshop Slideshows==<br />
<br />
[https://www.dropbox.com/s/lw5zpzhuvtb0y7k/Schultz%20slides%20Neuronex%202020.pdf?dl=0 Multiphoton imaging of hippocampal place cell network activity in mouse models of neurodegenerative disease - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1SOMoVpL8QR2k_om3BXadlJlyT3lIdbMg/view?usp=sharing Open Platforms for Two- and Three-Photon Microscopy - Rick Ayer]<br />
<br />
[https://www.dropbox.com/s/09s1gnf89cm90pb/2020_02_26_Nemonic%20copy.key?dl=0 Fast and scalable brain imaging data at single-cell resolution - Andrea Giovannucci]<br />
<br />
[https://www.dropbox.com/s/63lvmxaiwjmk8wh/Nemonic%20Seminar.pptx?dl=0 3D-SHOT with Computer Generated Holography at high speed and with high performance - Nicolas Pegard]<br />
<br />
[https://drive.google.com/file/d/1d65jhaoFW_MPfu-MVc6T8Qll35RUdudB/view?usp=sharing Advances in two-dimensional spatial frequency modulation imaging - Jeff Squier]<br />
<br />
==2020 Related Resources==<br />
<br />
https://www.youtube.com/watch?v=DLTaqzz8hJM&feature=youtu.be<br />
<br />
== 2019 Speakers ==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
Image:Kaspar_Podgorski.jpg|Kaspar Podgorski, Janelia Research Campus<br />
Image:Nico_Pegard.jpg|Nico Pégard, UNC Chapel Hill<br />
Image:Darcy_Peterka.jpg|Darcy Peterka, Columbia<br />
Image:Emily_Gibson.jpg|Emily Gibson, CU Denver<br />
Image:Andrea.png|Andrea Giovannucci, UNC Chapel Hill<br />
Image:Simon.jpg|Simon Schultz, Imperial College London<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
<br />
</gallery><br />
<br />
<br clear=all><br />
<br />
== 2019 Workshop Slideshows ==<br />
<br />
[https://drive.google.com/file/d/1d51qP8bYEYDuoxELvALhJHYg8EkmXgPB/view?usp=sharing Fast and scalable calcium imaging data analysis with CaImAn - Andrea Giovannucci]<br />
<br />
[https://drive.google.com/file/d/1ODxntA7qm54joT4Pebk6pZisuBVyeFdV/view?usp=sharing From Holography to 3D-SHOT Multiphoton whole-cell photostimulation in 3D With single neuron resolution and millisecond precision - Nico Pegard]<br />
<br />
[https://drive.google.com/file/d/1jaftx6n5dvVkoM_oFKTdjJgI-QKQIgsC/view?usp=sharing Robotically automated, two-photon targeted patch clamp physiology - Simon Schultz]<br />
<br />
[https://drive.google.com/file/d/1yfxZpgaVVFpYPj7DnJU07u6mvcnCwACi/view?usp=sharing High Speed Two-Photon Tomography - Kaspar Podgorski]<br />
<br />
[https://drive.google.com/file/d/17bMJbctNsF2DQNLSYF__1R5aKk-s7G4O/view?usp=sharing Extending multiphoton microscopy to 3D brain imaging in freely moving mice - Emily Gibson]<br />
<br />
[https://drive.google.com/file/d/1Cr_jK-VSmIBfMrZjOpeZcNOnEt5SqqZM/view?usp=sharing Darcy Peterka's 2019 Nemonic workshop talk - Darcy Peterka]<br />
<br />
== 2019 Related Resources==<br />
<br />
[https://elifesciences.org/articles/38173 CaImAn: an open source tool for scalable calcium imaging data analysis]<br />
<br />
[https://www.sciencedirect.com/science/article/pii/S089662731730733X Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology]<br />
<br />
[https://journals.sagepub.com/doi/abs/10.1177/2398212818776561 Progress in automating patch clamp cellular physiology]<br />
<br />
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5661356/ ABLE: An Activity-Based Level Set Segmentation Algorithm for Two-Photon Calcium Imaging Data]<br />
<br />
[https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-8-3678 High speed functional imaging with source localized multifocal two-photon microscopy]<br />
<br />
[https://drive.google.com/file/d/1geiUITveydNTSxz2lFEHyazqjx9XdjUE/view?usp=sharing Building a two-photon microscope is easy]<br />
<br />
== See Also ==<br />
<br />
[[Big Brain]]<br />
<br />
<br clear=all></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Nemonic_draft.jpg&diff=594File:Nemonic draft.jpg2022-12-06T22:08:37Z<p>Ryan.McGreal: </p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Maps&diff=593Maps2022-10-06T20:35:18Z<p>Ryan.McGreal: Created page with "=== Map of institutions === {{Location map+|United States|caption=A map of all tier one research universities in the contiguous United States. The University of Hawaii at Ma..."</p>
<hr />
<div>=== Map of institutions ===<br />
{{Location map+|United States|caption=A map of all tier one research universities in the contiguous United States. The [[University of Hawaii at Manoa]] is the only R1 institution that is not pictured. Blue dots represent [[Public university|public]] institutions whereas red dots represent [[Private university|private]] institutions.|float=center|width=750|places=<!-- Alabama --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UAB|University of Alabama at Birmingham}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.502123<br />
| lon_deg = -86.806445<br />
}} <br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|AU|Auburn University}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 32.3552<br />
| lon_deg = -85.2551<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UA|University of Alabama}}}}<br />
| mark = Blue pog.svg <br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.2098<br />
| lon_deg = -87.5692<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UAH|University of Alabama in Huntsville}}}}<br />
| mark = Blue pog.svg <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.73<br />
| lon_deg = -86.585<br />
}}<br />
<!-- Arkansas --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UA|University of Arkansas}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 36.067832<br />
| lon_deg = -94.173655<br />
}}<br />
<!-- Arizona --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|ASU|Arizona State University}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.424240<br />
| lon_deg = -111.928053<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UA|University of Arizona}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 32.231885<br />
| lon_deg = -110.950109<br />
}}<br />
<!-- California --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCD|University of California, Davis}}}}<br />
| mark = Blue pog.svg <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.538232<br />
| lon_deg = -121.761712<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCB|University of California, Berkeley}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 37.871899<br />
| lon_deg = -122.25854<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Stanford|Stanford University}}}}<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 37.427475<br />
| lon_deg = -122.169719<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCSC|University of California, Santa Cruz}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 36.991474<br />
| lon_deg = -122.058297<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCSB|University of California, Santa Barbara}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.413963<br />
| lon_deg = -119.848947<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCLA/USC|University of California, Los Angeles/University of Southern California}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.068921<br />
| lon_deg = -118.445181<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| position = <br />
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| link = <br />
| lat_deg = 34.022352<br />
| lon_deg = -118.285117<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCI|University of California, Irvine}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.640495<br />
| lon_deg = -117.844296<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Caltech|California Institute of Technology}}}}<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.137658<br />
| lon_deg = -118.125269<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCR|University of California, Riverside}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.973706<br />
| lon_deg = -117.328064<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCSD|University of California, San Diego}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 32.880060<br />
| lon_deg = -117.234014<br />
}}<br />
<!-- Colorado --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|CSU|Colorado State University}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.573436<br />
| lon_deg = -105.086547<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|CU Boulder|University of Colorado Boulder}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.007581<br />
| lon_deg = -105.265942<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|DU/CU Denver|University of Denver/University of Colorado Denver}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.742043<br />
| lon_deg = -104.991531<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|CSM|Colorado School of Mines}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.751093<br />
| lon_deg = -105.223268<br />
}}<br />
<!-- Connecticut --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UConn|University of Connecticut}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.807741<br />
| lon_deg = -72.253981<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Yale|Yale University}}}}<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.316324<br />
| lon_deg = -72.922343<br />
}}<br />
<!-- Washington, D.C. --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|GW/Gtwn.|George Washington University/Georgetown University}}}}<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.899715<br />
| lon_deg = -77.048599<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.907609<br />
| lon_deg = -77.072258<br />
}}<br />
<!-- Delaware --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UD|University of Delaware}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.677950<br />
| lon_deg = -75.750611<br />
}}<br />
<!-- Florida --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|FIU|Florida International University}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 25.756576<br />
| lon_deg = -80.373949<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|FSU|Florida State University}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 30.441878<br />
| lon_deg = -84.298489<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UF|University of Florida}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 29.643632<br />
| lon_deg = -82.354930<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UCF|University of Central Florida}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 28.602427<br />
| lon_deg = -81.200060<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|USF|University of South Florida}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 28.058703<br />
| lon_deg = -82.413854<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Miami|University of Miami}}}}<br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 25.719168<br />
| lon_deg = -80.277125<br />
}}<br />
<!-- Georgia --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Emory/GA Tech/GSU|Emory University/Georgia Institute of Technology/Georgia State University}}}}<br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.792519<br />
| lon_deg = -84.323999<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = Blue pog.svg<br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.753068<br />
| lon_deg = -84.385282<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.775618<br />
| lon_deg = -84.396285<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UGA|University of Georgia}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.948005<br />
| lon_deg = -83.377322<br />
}}<br />
<!-- Illinois --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|NU|Northwestern University}}}}<br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.056459<br />
| lon_deg = -87.675267<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UChicago/UIC|University of Chicago/University of Illinois at Chicago}}}}<br />
| mark = <br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.788608<br />
| lon_deg = -87.598713<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.869304<br />
| lon_deg = -87.647450<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UIUC|University of Illinois at Urbana–Champaign}}}}<br />
| mark = Blue pog.svg<br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.101952<br />
| lon_deg = -88.227161<br />
}}<br />
<!-- Indiana --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|IU|Indiana University Bloomington}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.174570<br />
| lon_deg = -86.512946<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Purdue|Purdue University}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.423705<br />
| lon_deg = -86.921195<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|ND|University of Notre Dame}}}}<br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.705572<br />
| lon_deg = -86.235339<br />
}}<br />
<!-- Iowa --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UIowa|University of Iowa}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 41.662696<br />
| lon_deg = -91.554900<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|ISU|Iowa State University}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.026619<br />
| lon_deg = -93.646465<br />
}}<br />
<!-- Kansas --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|KU|University of Kansas}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.954344<br />
| lon_deg = -95.255796<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|KSU|Kansas State University}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.197444<br />
| lon_deg = -96.584725<br />
}}<br />
<!-- Kentucky --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UK|University of Kentucky}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.030651<br />
| lon_deg = -84.503970<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UofL|University of Louisville}}}}<br />
| mark = Blue pog.svg<br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.212276<br />
| lon_deg = -85.758502<br />
}}<br />
<!-- Louisiana --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|ULL|University of Louisiana at Lafayette}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 30.211990<br />
| lon_deg = -92.019264<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|LSU|Louisiana State University}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 30.413258<br />
| lon_deg = -91.180002<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Tulane|Tulane University}}}}<br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 29.940348<br />
| lon_deg = -90.120728<br />
}}<br />
<!-- Maine --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMaine|University of Maine}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 44.99<br />
| lon_deg = -68.5568495<br />
}}<br />
<!-- Maryland --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|JHU|Johns Hopkins University}}}}<br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.329901<br />
| lon_deg = -76.620518<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMBC|University of Maryland, Baltimore County}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.2555<br />
| lon_deg = -76.711256<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMD|University of Maryland, College Park}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.986918<br />
| lon_deg = -76.942554<br />
}}<br />
<!-- Massachusetts --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMass|University of Massachusetts Amherst}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.391157<br />
| lon_deg = -72.526712<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|BC/BU/Hvd/MIT/NEU/Tfts|Boston College/Boston University/Harvard University/Massachusetts Institute of Technology/Northeastern University/Tufts University}}}}<br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.335549<br />
| lon_deg = -71.168495<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.350500<br />
| lon_deg = -71.105399<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.377003<br />
| lon_deg = -71.116660<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = <br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.360091<br />
| lon_deg = -71.094160<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = <br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.339807<br />
| lon_deg = -71.089172<br />
}}<br />
{{Location map~ | United States<br />
| label = <br />
| mark = <br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.364989<br />
| lon_deg = -71.258663<br />
}}<br />
{{Location map~ | United States<br />
| label =<br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.407484<br />
| lon_deg = -71.119023<br />
}}<br />
<!-- Michigan --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|WSU|Wayne State University}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.359139<br />
| lon_deg = -83.066546<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMich|University of Michigan}}}}<br />
| mark = Blue pog.svg<br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.278044<br />
| lon_deg = -83.738224<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|MI SU|Michigan State University}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.701848<br />
| lon_deg = -84.482172<br />
}}<br />
<!-- Minnesota --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UMN|University of Minnesota}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 44.973990<br />
| lon_deg = -93.227729<br />
}}<br />
<!-- Missouri --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|MU|University of Missouri}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.940381<br />
| lon_deg = -92.327738<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|WUSTL|Washington University in St. Louis}}}}<br />
| mark = <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 38.648789<br />
| lon_deg = -90.310796<br />
}}<br />
<!-- Mississippi --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|OleMiss|University of Mississippi}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.364732<br />
| lon_deg = -89.538443<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|USM|University of Southern Mississippi}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 31.3271<br />
| lon_deg = -89.2903<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|MS SU|Mississippi State University}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 33.4504<br />
| lon_deg = -88.8184<br />
}}<br />
<!-- Montana --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UM|The University of Montana}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = https://www.umt.edu<br />
| lat_deg = 46.872146<br />
| lon_deg = -113.9939982<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|MT SU|Montana State University}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 45.6674<br />
| lon_deg = -111.0546<br />
}}<br />
<!-- Nebraska --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UNL|University of Nebraska–Lincoln}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.820197<br />
| lon_deg = -96.700476<br />
}}<br />
<!-- Nevada --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UNR|University of Nevada, Reno}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 39.5442<br />
| lon_deg = -119.8164<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UNLV|University of Nevada, Las Vegas}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 36.1085<br />
| lon_deg = -115.1432<br />
}}<br />
<!-- New Hampshire --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Dartmouth|Dartmouth College}}}}<br />
| mark = <br />
| position =<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 43.7044<br />
| lon_deg = -72.2887<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UNH|University of New Hampshire}}}}<br />
| mark = Blue pog.svg<br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 43.1339<br />
| lon_deg = -70.9269<br />
}}<br />
<!-- New Jersey --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|NJIT|New Jersey Institute of Technology}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.743057<br />
| lon_deg = -74.178936<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Rutgers|Rutgers University–New Brunswick}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.500819<br />
| lon_deg = -74.447399<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Princeton|Princeton University}}}}<br />
| mark = <br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.343989<br />
| lon_deg = -74.651448<br />
}}<br />
<!-- New Mexico --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UNM|University of New Mexico}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 35.084319<br />
| lon_deg = -106.619781<br />
}}<br />
<!-- New York --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|UB|University at Buffalo}}}}<br />
| mark = Blue pog.svg<br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 43.000809<br />
| lon_deg = -78.788970<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Bing.|Binghamton University}}}}<br />
| mark = Blue pog.svg<br />
| position = bottom<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.08782<br />
| lon_deg = -75.96692<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|SUNY|University at Albany, SUNY}}}}<br />
| mark = Blue pog.svg<br />
| position = left<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.685888<br />
| lon_deg = -73.824347<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|SBU|Stony Brook University}}}}<br />
| mark = Blue pog.svg<br />
| position = right<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 40.912376<br />
| lon_deg = -73.123389<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Syr.|Syracuse University}}}}<br />
| mark = <br />
| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 43.039153<br />
| lon_deg = -76.135116<br />
}}<br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Cor.|Cornell University}}}}<br />
| mark = <br />
| position = <br />
| marksize = 5<br />
| link = <br />
| lat_deg = 42.453449<br />
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<!-- North Carolina --><br />
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<!-- Oklahoma --><br />
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<!-- Rhode Island --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Brown|Brown University}}}}<br />
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<!-- South Carolina --><br />
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| mark = Blue pog.svg<br />
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| label = {{small|{{tooltip|Clemson|Clemson University}}}}<br />
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| position = top<br />
| marksize = 5<br />
| link = <br />
| lat_deg = 34.676094<br />
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}}<br />
<!-- Tennessee --><br />
{{Location map~ | United States<br />
| label = {{small|{{tooltip|Memphis|University of Memphis}}}}<br />
| mark = Blue pog.svg<br />
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| marksize = 5<br />
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| lat_deg = 35.118458<br />
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| mark = Blue pog.svg<br />
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| label = {{small|{{tooltip|VU|Vanderbilt University}}}}<br />
| mark = <br />
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}}<br />
<!-- Texas --><br />
{{Location map~ | United States<br />
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| lat_deg = 31.550434<br />
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| label = {{small|{{tooltip|Rice|Rice University}}}}<br />
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| lat_deg = 29.717394<br />
| lon_deg = -95.401831<br />
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| label = {{small|{{tooltip|UT-Austin|University of Texas at Austin}}}}<br />
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<!-- Wisconsin --><br />
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| label = {{small|{{tooltip|UWMad|University of Wisconsin–Madison}}}}<br />
| mark = Blue pog.svg<br />
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| label = {{small|{{tooltip|UWMil|University of Wisconsin–Milwaukee}}}}<br />
| mark = Blue pog.svg<br />
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<!-- West Virginia --><br />
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}}}}{{clear}}</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=592Diesel user group2022-09-24T04:02:15Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]<br />
<br />
<br />
==BRAIN Initiative Alliance Toolmakers Resources Web Page==<br />
<br />
https://www.braininitiative.org/toolmakers/resources/dual-independent-enhanced-scan-engines-for-large-field-of-view-two-photon-imaging-diesel2p/</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=591Diesel user group2022-09-08T00:45:49Z<p>Ryan.McGreal: /* Overview of specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=590Diesel user group2022-09-08T00:44:16Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view: ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=589Diesel user group2022-09-08T00:43:05Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=588Diesel user group2022-09-08T00:42:14Z<p>Ryan.McGreal: /* Overview of specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=587Diesel user group2022-09-08T00:41:01Z<p>Ryan.McGreal: /* Overview of specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture <br />
<br />
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel microscope (2 per scan engine)<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=586Diesel user group2022-09-08T00:40:08Z<p>Ryan.McGreal: /* Overview of specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=585Diesel user group2022-09-08T00:39:55Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=584Diesel user group2022-09-08T00:27:11Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-Scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=583Diesel user group2022-09-07T20:56:18Z<p>Ryan.McGreal: /* Users */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=582Diesel user group2022-09-07T20:55:49Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
<br />
==Lens prescriptions==<br />
<br />
[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full Diesel2p lens prescription available here]</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=581Diesel user group2022-09-07T20:27:08Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
<br />
==Overview of specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
-[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full lens prescription available here]<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery><br />
<br />
==Publications==<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)</div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=580Diesel user group2022-09-07T20:24:31Z<p>Ryan.McGreal: /* Specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
-[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf Full lens prescription available here]<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=579Diesel user group2022-09-07T20:23:14Z<p>Ryan.McGreal: /* Specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
-[https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf|Full lens prescription available here]<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=578Diesel user group2022-09-07T20:22:56Z<p>Ryan.McGreal: /* Specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
-[Full lens prescription available here|https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf]<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=577Diesel user group2022-09-07T20:21:07Z<p>Ryan.McGreal: /* Specifications */</p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
'''Diesel2p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
<br />
-[Full lens prescription available here https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf]<br />
<br />
'''Quadroscope'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
<br />
-Dual independent scanning engines<br />
<br />
-Large field of view : ~5 x 5 mm2<br />
<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=576Diesel user group2022-09-07T20:20:31Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
'''Diesel2p'''<br />
-Dual independent scanning engines<br />
-Large field of view : ~5 x 5 mm2, encompassing distances up to 7 mm<br />
-scan angles up to ±5 degrees at the objective back aperture and primarily for the excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm<br />
-[Full lens prescription available here https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-26736-4/MediaObjects/41467_2021_26736_MOESM1_ESM.pdf]<br />
<br />
'''Quadroscope'''<br />
-Dual independent scanning engines<br />
-Large field of view : ~5 x 5 mm2<br />
-4-channel miscoscope (2 per scan engine)<br />
<br />
'''Diesel3p'''<br />
-Dual independent scanning engines<br />
-Large field of view : ~5 x 5 mm2<br />
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm<br />
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective <br />
<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=575Diesel user group2022-09-07T19:18:27Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br clear=all><br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Specifications==<br />
<br />
<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=574Diesel user group2022-09-07T19:17:28Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
[[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] <br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:D3p.png&diff=573File:D3p.png2022-09-07T19:16:35Z<p>Ryan.McGreal: </p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=572Diesel user group2022-09-07T19:15:51Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
[[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Quadroscope.png&diff=571File:Quadroscope.png2022-09-07T19:14:41Z<p>Ryan.McGreal: </p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=570Diesel user group2022-09-07T19:13:49Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=569Diesel user group2022-09-07T19:13:16Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|600px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|620px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=568Diesel user group2022-09-07T19:13:05Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|600px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|640px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=567Diesel user group2022-09-07T19:12:44Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|600px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|600px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=566Diesel user group2022-09-07T19:12:28Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br clear=all><br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|640px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|640px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br clear=all><br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=565Diesel user group2022-09-07T19:11:46Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|640px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|640px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=564Diesel user group2022-09-07T19:11:31Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|640px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:D2p_2.png|640px|thumb|right|caption|Diesel2p system layout]]<br />
<br />
<br />
<br />
<br />
[[File:D2p.png|thumb|640px]] '''a''' Functional cortical areas in the mouse brain are widely distributed. A field-of-view (FOV) of Ø5 mm can encompass multiple brain areas, and independent scan engines can capture ongoing neural activity in multiple cortical areas simultaneously with optimized scan parameters. '''b''' Two imaging beams are temporally multiplexed and independently positioned in XY using two sets of resonant-galvo-galvo scan engines. First, overall power is attenuated using a half-wave plate (λ/2 WP) and a polarizing beam splitting cube (PBS). A 2X beam expander (1:2 BE) enlarges the beam for the clear aperture of the deformable mirrors adaptive optics (AO). A custom single-prism pre-chirper offsets system dispersion to maintain transform-limited pulses at the focal plane. A second λ/2 WP and PBS pair divides the beam into two pathways. Pathway 2 (s-polarization in orange) passes to a delay arm where it travels 1.87 m further than Pathway 1 using mirrors, thus delaying it by 6.25 ns relative to Pathway 1 (p-polarization in blue). Both pathways each proceed to deformable mirrors for adjusting the focal plane and correcting optical aberrations before being directed to resonant-galvo-galvo scan engines. All scanning mirrors are optically relayed to each other. Each pathway then passes through a scan lens before being combined with a beam recombination relay. A tube lens and an infrared-reflective dichroic mirror relay the two multiplexed beams onto the back aperture of the objective. Fluorescence (green) is directed to a photomultiplier tube (PMT) via an assembly of collection lenses (CL1, CL2, CL3). '''c''' An oblique view of a 3-D model of the system and its footprint. '''d''' A top view with the arrangement of the two scan engines highlighted. '''e''' Plot of the model Strehl ratio across the scan area indicates diffraction limited performance (Strehl ratio > 0.8) across ~25 mm2, significantly larger than the area of the dashed 5-mm diameter circle (~19.6 mm2) by ~28%. '''f''' Multiphoton excitation PSF measurements were made with subresolution beads (0.2 µm) in agar under a coverslip at three depths and four locations, for both of the AO-equipped, temporally multiplexed beam pathways. FWHM of the Gaussian fits for measurements from the fluorescence beads radially and axially are calculated and plotted. Eight beads (n = 8) at each locations are measured, except that there are 7 beads (n = 7) measured on axis at the depth of 500 µm. Data are presented as mean values ± S.D. '''g''' XY images of a calibrated, structured fluorescent sample with a periodic line pattern (5 lines per millimeter) in two orientations acquired under the full scan range of the system. Each image shows 25 lines on the top edge (left image) and on the left edge (right image), receptively, verifying a 5 × 5 mm FOV. '''h''' The XZ image along the orange dashed line and the YZ image along green dashed line in (g) are also plotted. The imaging pattern is colinear with the straight lines, suggesting a flat field both in x and y directions across the FOV.<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:D2p_2.png&diff=563File:D2p 2.png2022-09-07T19:10:29Z<p>Ryan.McGreal: </p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=562Diesel user group2022-09-07T19:09:47Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|640px|thumb|left|caption|Diesel2p objective and stage]]<br />
[[File:Blue-fullview-fisheye.jpg|640px|thumb|right|caption| ]]<br />
<br />
<br />
<br />
<br />
[[File:D2p.png|thumb|640px]] '''a''' Functional cortical areas in the mouse brain are widely distributed. A field-of-view (FOV) of Ø5 mm can encompass multiple brain areas, and independent scan engines can capture ongoing neural activity in multiple cortical areas simultaneously with optimized scan parameters. '''b''' Two imaging beams are temporally multiplexed and independently positioned in XY using two sets of resonant-galvo-galvo scan engines. First, overall power is attenuated using a half-wave plate (λ/2 WP) and a polarizing beam splitting cube (PBS). A 2X beam expander (1:2 BE) enlarges the beam for the clear aperture of the deformable mirrors adaptive optics (AO). A custom single-prism pre-chirper offsets system dispersion to maintain transform-limited pulses at the focal plane. A second λ/2 WP and PBS pair divides the beam into two pathways. Pathway 2 (s-polarization in orange) passes to a delay arm where it travels 1.87 m further than Pathway 1 using mirrors, thus delaying it by 6.25 ns relative to Pathway 1 (p-polarization in blue). Both pathways each proceed to deformable mirrors for adjusting the focal plane and correcting optical aberrations before being directed to resonant-galvo-galvo scan engines. All scanning mirrors are optically relayed to each other. Each pathway then passes through a scan lens before being combined with a beam recombination relay. A tube lens and an infrared-reflective dichroic mirror relay the two multiplexed beams onto the back aperture of the objective. Fluorescence (green) is directed to a photomultiplier tube (PMT) via an assembly of collection lenses (CL1, CL2, CL3). '''c''' An oblique view of a 3-D model of the system and its footprint. '''d''' A top view with the arrangement of the two scan engines highlighted. '''e''' Plot of the model Strehl ratio across the scan area indicates diffraction limited performance (Strehl ratio > 0.8) across ~25 mm2, significantly larger than the area of the dashed 5-mm diameter circle (~19.6 mm2) by ~28%. '''f''' Multiphoton excitation PSF measurements were made with subresolution beads (0.2 µm) in agar under a coverslip at three depths and four locations, for both of the AO-equipped, temporally multiplexed beam pathways. FWHM of the Gaussian fits for measurements from the fluorescence beads radially and axially are calculated and plotted. Eight beads (n = 8) at each locations are measured, except that there are 7 beads (n = 7) measured on axis at the depth of 500 µm. Data are presented as mean values ± S.D. '''g''' XY images of a calibrated, structured fluorescent sample with a periodic line pattern (5 lines per millimeter) in two orientations acquired under the full scan range of the system. Each image shows 25 lines on the top edge (left image) and on the left edge (right image), receptively, verifying a 5 × 5 mm FOV. '''h''' The XZ image along the orange dashed line and the YZ image along green dashed line in (g) are also plotted. The imaging pattern is colinear with the straight lines, suggesting a flat field both in x and y directions across the FOV.<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Blue-fullview-fisheye.jpg&diff=561File:Blue-fullview-fisheye.jpg2022-09-07T19:05:32Z<p>Ryan.McGreal: Ryan.McGreal uploaded a new version of File:Blue-fullview-fisheye.jpg</p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Blue-oblique-fisheye.jpg&diff=560File:Blue-oblique-fisheye.jpg2022-09-07T19:03:30Z<p>Ryan.McGreal: Ryan.McGreal uploaded a new version of File:Blue-oblique-fisheye.jpg</p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=559Diesel user group2022-09-07T19:01:49Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|640px]] diesel2p objective and stage<br />
<br />
<br />
<br />
<br />
[[File:D2p.png|thumb|640px]] '''a''' Functional cortical areas in the mouse brain are widely distributed. A field-of-view (FOV) of Ø5 mm can encompass multiple brain areas, and independent scan engines can capture ongoing neural activity in multiple cortical areas simultaneously with optimized scan parameters. '''b''' Two imaging beams are temporally multiplexed and independently positioned in XY using two sets of resonant-galvo-galvo scan engines. First, overall power is attenuated using a half-wave plate (λ/2 WP) and a polarizing beam splitting cube (PBS). A 2X beam expander (1:2 BE) enlarges the beam for the clear aperture of the deformable mirrors adaptive optics (AO). A custom single-prism pre-chirper offsets system dispersion to maintain transform-limited pulses at the focal plane. A second λ/2 WP and PBS pair divides the beam into two pathways. Pathway 2 (s-polarization in orange) passes to a delay arm where it travels 1.87 m further than Pathway 1 using mirrors, thus delaying it by 6.25 ns relative to Pathway 1 (p-polarization in blue). Both pathways each proceed to deformable mirrors for adjusting the focal plane and correcting optical aberrations before being directed to resonant-galvo-galvo scan engines. All scanning mirrors are optically relayed to each other. Each pathway then passes through a scan lens before being combined with a beam recombination relay. A tube lens and an infrared-reflective dichroic mirror relay the two multiplexed beams onto the back aperture of the objective. Fluorescence (green) is directed to a photomultiplier tube (PMT) via an assembly of collection lenses (CL1, CL2, CL3). '''c''' An oblique view of a 3-D model of the system and its footprint. '''d''' A top view with the arrangement of the two scan engines highlighted. '''e''' Plot of the model Strehl ratio across the scan area indicates diffraction limited performance (Strehl ratio > 0.8) across ~25 mm2, significantly larger than the area of the dashed 5-mm diameter circle (~19.6 mm2) by ~28%. '''f''' Multiphoton excitation PSF measurements were made with subresolution beads (0.2 µm) in agar under a coverslip at three depths and four locations, for both of the AO-equipped, temporally multiplexed beam pathways. FWHM of the Gaussian fits for measurements from the fluorescence beads radially and axially are calculated and plotted. Eight beads (n = 8) at each locations are measured, except that there are 7 beads (n = 7) measured on axis at the depth of 500 µm. Data are presented as mean values ± S.D. '''g''' XY images of a calibrated, structured fluorescent sample with a periodic line pattern (5 lines per millimeter) in two orientations acquired under the full scan range of the system. Each image shows 25 lines on the top edge (left image) and on the left edge (right image), receptively, verifying a 5 × 5 mm FOV. '''h''' The XZ image along the orange dashed line and the YZ image along green dashed line in (g) are also plotted. The imaging pattern is colinear with the straight lines, suggesting a flat field both in x and y directions across the FOV.<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=558Diesel user group2022-09-07T19:01:36Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg]] diesel2p objective and stage<br />
<br />
<br />
<br />
<br />
[[File:D2p.png|thumb|640px]] '''a''' Functional cortical areas in the mouse brain are widely distributed. A field-of-view (FOV) of Ø5 mm can encompass multiple brain areas, and independent scan engines can capture ongoing neural activity in multiple cortical areas simultaneously with optimized scan parameters. '''b''' Two imaging beams are temporally multiplexed and independently positioned in XY using two sets of resonant-galvo-galvo scan engines. First, overall power is attenuated using a half-wave plate (λ/2 WP) and a polarizing beam splitting cube (PBS). A 2X beam expander (1:2 BE) enlarges the beam for the clear aperture of the deformable mirrors adaptive optics (AO). A custom single-prism pre-chirper offsets system dispersion to maintain transform-limited pulses at the focal plane. A second λ/2 WP and PBS pair divides the beam into two pathways. Pathway 2 (s-polarization in orange) passes to a delay arm where it travels 1.87 m further than Pathway 1 using mirrors, thus delaying it by 6.25 ns relative to Pathway 1 (p-polarization in blue). Both pathways each proceed to deformable mirrors for adjusting the focal plane and correcting optical aberrations before being directed to resonant-galvo-galvo scan engines. All scanning mirrors are optically relayed to each other. Each pathway then passes through a scan lens before being combined with a beam recombination relay. A tube lens and an infrared-reflective dichroic mirror relay the two multiplexed beams onto the back aperture of the objective. Fluorescence (green) is directed to a photomultiplier tube (PMT) via an assembly of collection lenses (CL1, CL2, CL3). '''c''' An oblique view of a 3-D model of the system and its footprint. '''d''' A top view with the arrangement of the two scan engines highlighted. '''e''' Plot of the model Strehl ratio across the scan area indicates diffraction limited performance (Strehl ratio > 0.8) across ~25 mm2, significantly larger than the area of the dashed 5-mm diameter circle (~19.6 mm2) by ~28%. '''f''' Multiphoton excitation PSF measurements were made with subresolution beads (0.2 µm) in agar under a coverslip at three depths and four locations, for both of the AO-equipped, temporally multiplexed beam pathways. FWHM of the Gaussian fits for measurements from the fluorescence beads radially and axially are calculated and plotted. Eight beads (n = 8) at each locations are measured, except that there are 7 beads (n = 7) measured on axis at the depth of 500 µm. Data are presented as mean values ± S.D. '''g''' XY images of a calibrated, structured fluorescent sample with a periodic line pattern (5 lines per millimeter) in two orientations acquired under the full scan range of the system. Each image shows 25 lines on the top edge (left image) and on the left edge (right image), receptively, verifying a 5 × 5 mm FOV. '''h''' The XZ image along the orange dashed line and the YZ image along green dashed line in (g) are also plotted. The imaging pattern is colinear with the straight lines, suggesting a flat field both in x and y directions across the FOV.<br />
<br />
==Publications==<br />
<br />
<br />
===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
<br />
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
<br />
===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
<br />
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
<br />
==Users==<br />
<br />
<gallery widths=300px heights=200px mode="nolines"><br />
<br />
Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
<br />
</gallery></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Blue-oblique-fisheye.jpg&diff=557File:Blue-oblique-fisheye.jpg2022-09-07T19:01:06Z<p>Ryan.McGreal: Ryan.McGreal uploaded a new version of File:Blue-oblique-fisheye.jpg</p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=File:Blue-oblique-fisheye.jpg&diff=556File:Blue-oblique-fisheye.jpg2022-09-07T19:00:34Z<p>Ryan.McGreal: </p>
<hr />
<div></div>Ryan.McGrealhttps://nemonic.ece.ucsb.edu/index.php?title=Diesel_user_group&diff=555Diesel user group2022-09-07T18:57:57Z<p>Ryan.McGreal: </p>
<hr />
<div><br />
Under Construction<br />
<br />
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems<br />
<br />
==What is Diesel?==<br />
<br />
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independ scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is currently operated by the Kara lab. <br />
<br />
[[File:Blue-fullview-fisheye.jpg|1280px]] diesel2p objective and stage<br />
<br />
<br />
<br />
<br />
[[File:D2p.png|thumb|640px]] '''a''' Functional cortical areas in the mouse brain are widely distributed. A field-of-view (FOV) of Ø5 mm can encompass multiple brain areas, and independent scan engines can capture ongoing neural activity in multiple cortical areas simultaneously with optimized scan parameters. '''b''' Two imaging beams are temporally multiplexed and independently positioned in XY using two sets of resonant-galvo-galvo scan engines. First, overall power is attenuated using a half-wave plate (λ/2 WP) and a polarizing beam splitting cube (PBS). A 2X beam expander (1:2 BE) enlarges the beam for the clear aperture of the deformable mirrors adaptive optics (AO). A custom single-prism pre-chirper offsets system dispersion to maintain transform-limited pulses at the focal plane. A second λ/2 WP and PBS pair divides the beam into two pathways. Pathway 2 (s-polarization in orange) passes to a delay arm where it travels 1.87 m further than Pathway 1 using mirrors, thus delaying it by 6.25 ns relative to Pathway 1 (p-polarization in blue). Both pathways each proceed to deformable mirrors for adjusting the focal plane and correcting optical aberrations before being directed to resonant-galvo-galvo scan engines. All scanning mirrors are optically relayed to each other. Each pathway then passes through a scan lens before being combined with a beam recombination relay. A tube lens and an infrared-reflective dichroic mirror relay the two multiplexed beams onto the back aperture of the objective. Fluorescence (green) is directed to a photomultiplier tube (PMT) via an assembly of collection lenses (CL1, CL2, CL3). '''c''' An oblique view of a 3-D model of the system and its footprint. '''d''' A top view with the arrangement of the two scan engines highlighted. '''e''' Plot of the model Strehl ratio across the scan area indicates diffraction limited performance (Strehl ratio > 0.8) across ~25 mm2, significantly larger than the area of the dashed 5-mm diameter circle (~19.6 mm2) by ~28%. '''f''' Multiphoton excitation PSF measurements were made with subresolution beads (0.2 µm) in agar under a coverslip at three depths and four locations, for both of the AO-equipped, temporally multiplexed beam pathways. FWHM of the Gaussian fits for measurements from the fluorescence beads radially and axially are calculated and plotted. Eight beads (n = 8) at each locations are measured, except that there are 7 beads (n = 7) measured on axis at the depth of 500 µm. Data are presented as mean values ± S.D. '''g''' XY images of a calibrated, structured fluorescent sample with a periodic line pattern (5 lines per millimeter) in two orientations acquired under the full scan range of the system. Each image shows 25 lines on the top edge (left image) and on the left edge (right image), receptively, verifying a 5 × 5 mm FOV. '''h''' The XZ image along the orange dashed line and the YZ image along green dashed line in (g) are also plotted. The imaging pattern is colinear with the straight lines, suggesting a flat field both in x and y directions across the FOV.<br />
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==Publications==<br />
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===Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry===<br />
https://doi.org/10.1038/s41467-021-26736-4<br />
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Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)<br />
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===Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds===<br />
https://doi.org/10.1038/s41467-021-26737-3<br />
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Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)<br />
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==Users==<br />
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<gallery widths=300px heights=200px mode="nolines"><br />
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Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara<br />
Image:Jerry_Chen_BU.jpg|Co-Principal Investigator: Jerry Chen, Boston University<br />
Image:Prakash_Kara_UMN.png|Co-Principal Investigator: Prakash Kara, University of Minnesota <br />
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</gallery></div>Ryan.McGreal