Diesel user group - Revision history

Ryan McGreal at 16:03, 6 June 2023

2023-06-06T16:03:09Z

Ryan McGreal at 16:02, 6 June 2023

2023-06-06T16:02:58Z

Ryan McGreal at 16:02, 6 June 2023

2023-06-06T16:02:32Z

Ryan McGreal at 14:55, 6 June 2023

2023-06-06T14:55:22Z

Ryan McGreal: /* What is DieselHub? */

2023-06-06T00:32:53Z

‎What is DieselHub?

Ryan McGreal at 00:29, 6 June 2023

2023-06-06T00:29:18Z

Ryan McGreal: /* What is DieselHub? */

2023-06-05T21:40:39Z

‎What is DieselHub?

Ryan McGreal: /* What is DieselHub? */

2023-06-05T21:38:59Z

‎What is DieselHub?

Ryan McGreal at 21:33, 5 June 2023

2023-06-05T21:33:51Z

Ryan McGreal at 19:16, 25 May 2023

2023-05-25T19:16:52Z

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 Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.
-This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems.
+Diesel2p is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems.
 We expect this important project to have a large positive impact on neuroscience research.

@@ Line 13: / Line 13: @@
 Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.
-In particular, we’re excited to see this being done with the Diesel2p platform. This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems.
+This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems.
 We expect this important project to have a large positive impact on neuroscience research.

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 DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens.
-Both models will be used to benefit the needs of diverse researchers nationwide. We are excited to see that the Smith lab is setting up such a dissemination hub. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.
+Both models will be used to benefit the needs of diverse researchers nationwide. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.
 In particular, we’re excited to see this being done with the Diesel2p platform. This platform is the most powerful and flexible large field-of-view imaging system to date. It has the largest field-of-view (25 mm^2) for accessing multiple brain regions simultaneously, dual independent scan engines for flexible imaging and optogenetic stimulation, and a long working (8 mm) distance air objective for easy implementation with a range of animals and behavior systems.

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 ==What is DieselHub?==
-DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community by making several Diesel2p microscopes available for community use.
+DieselHub will be an important, impactful project for the neuroscience community, accelerating research and supporting diverse researchers.
-Not only is multiphoton imaging equipment expensive, it requires expertise to operate that few labs have. Thus, even if imaging rigs are available at a university or institute, it is not as simple as going across the hall to use one as the expertise to use such technology is the second bottleneck, after the expense of equipment. These barriers disproportionately affect new labs as they cannot take the financial risk to purchase an imaging rig without pilot data, in addition to requiring the appropriate mentorship and training on the use of such technology.
+Large field-of-view multiphoton imaging and optogenetic activation systems enable a broad array of state-of-the-art neuroscience research. However, it is expensive, both in cost and human capital. These systems cost hundreds of thousands of dollars and to make the most of them requires high level expertise. Thus, it is not as simple as going across the hall and borrowing one. These cost and expertise barriers disproportionately affect new labs as they cannot take the financial risk to purchase a system without pilot data, and/or expertise. Thus, rapid technological advancements in systems neuroscience can remain out-of-reach of diverse and creative scientists nationwide.
-DieselHub will overcome these barriers with two models. First, at UCSB DieselHub will host a centralized hub similar to an observatory model used in astronomy. The instrumentation as well as the technical expertise will be hosted at UCSB, eliminating the barriers of both equipment expense and expertise to selected users. This model is similar to the successful model demonstrated by the OpenScope project at the Allen Institute for Brain Science.
+DieselHub will overcome these barriers with two models. First, at UCSB the team will host a centralized hub, similar to an observatory model used in astronomy (or the OpenScope project at the Allen Institute for Brain Science). The instrumentation and technical personnel will be hosted at UCSB, eliminating the barriers of both cost and expertise. Second, the team will create and support traveling instrumentation. DieselHub will set up state-of-the-art Diesel2p rigs at user institutes. The team will train personnel and provide support. This model is important for some animal species and/or experiments that entail long training regimens.
-The second model involves traveling instrumentation. DieselHub will set up our imaging rigs at selected user institutes and train personnel on the use of our rigs. By bringing the microscopes to the users, we will make certain experiments more practical by eliminating the need for users to travel to UCSB as well as eliminating the need for users to ship and retrain animals. This model is similar to how microscope companies set up demonstration equipment at user facilities so that researchers can use new state-of-the-art equipment, such as the Nikon BioImaging Centers and Olympus Discovery Centers.
+Both models will be used to benefit the needs of diverse researchers nationwide. We are excited to see that the Smith lab is setting up such a dissemination hub. The neuroscience community desperately needs community accessible, large field-of-view multiphoton systems for imaging and optogenetic manipulation, and DieselHub can address this need. The impact of this project will likely be felt across many subfields of systems neuroscience, and greatly benefit neuroscience researchers across career stages.
 ==What is Diesel2p?==

@@ Line 5: / Line 5: @@
 ==What is DieselHub?==
-DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community.
+DieselHub will address the lack of easily accessible multiphoton imaging technology in the neuroscience community by making several Diesel2p microscopes available for community use.
 Not only is multiphoton imaging equipment expensive, it requires expertise to operate that few labs have. Thus, even if imaging rigs are available at a university or institute, it is not as simple as going across the hall to use one as the expertise to use such technology is the second bottleneck, after the expense of equipment. These barriers disproportionately affect new labs as they cannot take the financial risk to purchase an imaging rig without pilot data, in addition to requiring the appropriate mentorship and training on the use of such technology.

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 This page is the home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems
 <br clear=all>
 ==What is Diesel2p?==
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 -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm
 ==Other Diesel Systems==

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 practical, such as animals that cannot be easily shipped, or require extensive training. A traveling imaging system can be shipped to where the animals are maintained, experts can provide training and support, and the imaging experiment can be performed on-site.
-Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.
+DieselHub will use both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.
 ==Other Diesel Systems==

← Older revision		Revision as of 21:33, 5 June 2023
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	==What is DieselHub?==		==What is DieselHub?==

		+	Cost and technical expertise are two key barriers to wider adoption of next-generation multiphoton imaging systems. A commercially available large FOV two-photon imaging system costs over 600,000 USD (ThorLabs), despite still lacking multi-region two-photon optogenetic stimulation capabilities. Even once a lab procures the funds, the systems require technical expertise to take full advantage of the technology and lead to good experiments and insights. These barriers are particularly acute for new labs that cannot take a risk on such a heavy investment without pilot data and training with the technology. To overcome these barriers, it is compelling to pursue alternative dissemination strategies. We propose to make Diesel2p systems available widely across the US, at little-to-no cost, and with expert guidance.
		+
		+	There are two existing models for technology dissemination that we can build upon. The first model is a centralized facility. Similar to observatories in the field of astronomy, the concept is to centralize the instrumentation and technical personnel, and solicit applications from external users. Recently, the Allen Institute for Brain Science launched the OpenScope project (U24NS113646). Studies are coming out from that project and neuroscience can benefit from spreading the model more broadly.
		+
		+	A second model is traveling instrumentation. There is a long tradition of microscope companies setting up demonstration facilities at universities so that researchers can learn about and use state-of-the-art equipment. For example, the Nikon BioImaging Centers and Olympus Discovery Centers are located at universities, providing access to state-of-the-art instrumentation. This model is particularly useful when centralized experiments are not
		+	practical, such as animals that cannot be easily shipped, or require extensive training. A traveling imaging system can be shipped to where the animals are maintained, experts can provide training and support, and the imaging experiment can be performed on-site.
		+
		+	Both models will be used to benefit the needs of researchers nationwide. DieselHub is not a regional dissemination mechanism, it will benefit labs and institutes nationwide. In addition, emphasis will be placed on selecting users that are outlined in the DieselHub Plan for Enhancing Diverse Perspectives.

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 ==What is Diesel2p?==
-Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab.
+Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines.
 [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]]
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 ==Other Diesel Systems==
 '''Quadroscope'''