Difference between revisions of "Diesel user group"
Ryan McGreal (talk | contribs) |
Ryan McGreal (talk | contribs) (→Other Diesel Systems) |
||
Line 31: | Line 31: | ||
'''Quadroscope''' | '''Quadroscope''' | ||
− | [[File:Quadroscope.png|thumb|600px | + | [[File:Quadroscope.png|thumb|600px|caption|Quadroscope: a 4-channel variant of Diesel2p]] |
-Dual independent scanning engines | -Dual independent scanning engines | ||
Line 44: | Line 44: | ||
'''Diesel3p''' | '''Diesel3p''' | ||
− | [[File:D3p.png|thumb|600px | + | [[File:D3p.png|thumb|600px|caption| Diesel3p: a three-photon Diesel variant]] |
-Dual independent scanning engines | -Dual independent scanning engines | ||
Line 53: | Line 53: | ||
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective | -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective | ||
− | |||
− | |||
==Publications== | ==Publications== |
Revision as of 11:50, 25 May 2023
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems
Contents
What is Diesel2p?
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the Smith lab. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab.
Overview of specifications
Diesel2p
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm
-Scan angles up to ±5 degrees at the objective back aperture
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm
Other Diesel Systems
Quadroscope
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2
-4-channel microscope (2 per scan engine)
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm
Diesel3p
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective
Publications
Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
https://doi.org/10.1038/s41467-021-26736-4
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)
Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
https://doi.org/10.1038/s41467-021-26737-3
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)
Lens prescriptions
Full Diesel2p lens prescription available here