Difference between revisions of "Diesel user group"
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This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems | This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems | ||
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| − | ==What is | + | ==What is Diesel2p?== |
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. | Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the [http://slslab.org/ Smith lab]. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab. | ||
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[[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]] | [[File:Blue-fullview-fisheye.jpg|thumb|600px|left|caption|Diesel2p objective and stage]] | ||
[[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]] | [[File:D2p_2.png|thumb|600px|right|caption|Diesel2p system layout]] | ||
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-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm | -Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm | ||
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| + | ==Other Diesel Systems== | ||
'''Quadroscope''' | '''Quadroscope''' | ||
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| + | [[File:Quadroscope.png|thumb|600px|left|caption|Quadroscope: a 4-channel variant of Diesel2p]] | ||
-Dual independent scanning engines | -Dual independent scanning engines | ||
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'''Diesel3p''' | '''Diesel3p''' | ||
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| + | [[File:D3p.png|thumb|600px|right|caption| Diesel3p: a three-photon Diesel variant]] | ||
-Dual independent scanning engines | -Dual independent scanning engines | ||
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-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective | -8-mm long working distance, 360 degree rotatable, 0.54NA, air objective | ||
| − | ==Users== | + | %==Users== |
| − | <gallery widths=300px heights=200px mode="nolines"> | + | %<gallery widths=300px heights=200px mode="nolines"> |
| − | Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara | + | %Image:Spencer_Smith_UCSB.jpg|Spencer Smith, UC Santa Barbara |
| − | Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University | + | %Image:Jerry_Chen_BU.jpg|Jerry Chen, Boston University |
| − | Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota | + | %Image:Prakash_Kara_UMN.png|Prakash Kara, University of Minnesota |
</gallery> | </gallery> | ||
Revision as of 11:50, 25 May 2023
This page will serve as a home for the users of Diesel (Dual Independent Enhanced Scan Engines for Large field-of-view) multiphoton imaging systems
Contents
What is Diesel2p?
Diesel2p (Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging) is a multiphoton imaging system designed by the Smith lab. With approximately a dozen systems currently in operation or ordered, this custom and fully open-sourced system allows for large field-of view-imaging with two independent scan engines. In addition to Diesel2p, a 4-channel version is in operation at the Chen lab and a three-photon version is operated by the Kara lab.
Overview of specifications
Diesel2p
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2, encompassing distances up to 7 mm
-Scan angles up to ±5 degrees at the objective back aperture
-Excitation windows of 910 ± 10 nm and 1050 nm ± 10 nm
Other Diesel Systems
Quadroscope
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2
-4-channel microscope (2 per scan engine)
-Diffraction-limited performance optimized across an excitation wavelength range of 920–1040 nm
Diesel3p
-Dual independent scanning engines
-Large field of view: ~5 x 5 mm2
-Allow simultaneous 2p + 3p imaging, achromatic at 920nm and 1300 nm
-8-mm long working distance, 360 degree rotatable, 0.54NA, air objective
%==Users==
%
- %Image:Spencer Smith UCSB.jpg
Spencer Smith, UC Santa Barbara
- %Image:Jerry Chen BU.jpg
Jerry Chen, Boston University
- %Image:Prakash Kara UMN.png
Prakash Kara, University of Minnesota
Publications
Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
https://doi.org/10.1038/s41467-021-26736-4
Yu, Che-Hang ; Stirman, Jeffrey N. ; Yu, Yiyi ; Hira, Riichiro ; Smith, Spencer L. ( November 2021 , Nature Communications)
Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
https://doi.org/10.1038/s41467-021-26737-3
Clough, Mitchell ; Chen, Ichun Anderson ; Park , Seong-Wook ; Ahrens , Allison M. ; Stirman, Jeffrey N.; Smith, Spencer L. ; Chen, Jerry L. ( November 2021 , Nature Communications)
Lens prescriptions
Full Diesel2p lens prescription available here
